What is the amount of liquid added to the cell culture erlenmeyer flask?

In suspension cell culture, cell culture erlenmeyer flask is commonly used as a cell culture consumable. The growth of suspension cells does not rely on the surface of the support and grows in a suspended state in the culture medium. In actual culture, how do we determine the amount of liquid to add?

The common specifications of cell culture erlenmeyer flask include 125ml, 250ml, 500ml, and 1000ml, which meet the needs of different scales of cell culture. For example, 125ml and 250ml small-capacity bottles are mainly used for small-scale experiments, while 500ml and 1000ml specifications are used for medium-scale cell culture experiments. When this consumable is used, the vibration of the shaker is used to reduce the clumping rate of cells and maintain a good growth state of cells. Cell culture needs to be carried out in a sterile environment. Therefore, the triangular culture flask will undergo special sterilization treatment before it is put into use to achieve the effect of no DNase, no RNase, and no animal-derived components, providing a good environment for cell growth. environment.

Cell Culture Erlenmeyer Flask

According to the different bottle specifications, the recommended liquid volume for the four specifications of the bottles from low to high is 30ml, 60ml, 125ml, 500ml respectively. Generally, the volume of the solution when cultivating cells is controlled at about 20%-30% of the total volume of the cell culture erlenmeyer flask, and there are clear scale lines on the bottle body, which is convenient for visual observation of the volume of the solution.

The above are the recommended liquid volumes for cell culture erlenmeyer flask of different specifications. This capacity is not fixed. It should be considered comprehensively according to cell growth characteristics and inoculation density, so as to avoid affecting cell growth due to excessive liquid volume.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.