What does non-pyrogenic cell culture flask mean?

Cell culture flasks are a kind of consumables commonly used in the process of cell culture. Cell growth has high requirements on the environment, and the selection of consumables must also meet the requirements of non-pyrogenic. So, what does non-pyrogenic cell culture flask mean?

There are two types of pyrogens, one is endogenous pyrogens, which act on the thermoregulatory center of the hypothalamus and can increase body temperature. One is exogenous pyrogens, which refer to foreign substances that can be used as the cause of fever. It usually works by inducing endogenous pyrogens. What we usually call cell culture flasks and other consumables without pyrogens refers to exogenous pyrogens.

Exogenous pyrogens are mainly bacterial pyrogens, which are metabolites of certain bacteria, bacterial corpses and endotoxins. The products of gram-negative bacilli have the strongest pyrogenic ability, followed by gram-positive bacilli, and gram-positive cocci are weaker. Molds, yeasts, and even viruses can also produce pyrogens. Pyrogens are usually complexes of phospholipid polyols combined with proteins. Phospholipid polyol is the active center of the complex and has the strongest pyrogenic effect. Its chemical composition varies from species to species. The molecular weight is 5 × 104 to 5 × 105, and the larger the molecular weight, the stronger the thermal effect. In the process of cell culture, if the consumables contain pyrogens, it will directly affect the growth and reproduction of cells, so these consumables must be specially treated to remove possible exogenous endotoxins and ensure the normal growth of cells.

In addition to cell culture flasks, consumables such as micropipettes and associated pipette tips used in cell culture require special treatment to eliminate pyrogens before they can be put into use.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.