English
In the cell factory analogy for large-scale cell culture, different solutions play pivotal roles in supporting cell growth and function. Here, we discuss three critical solutions that are commonly used in cell culture: PBS buffer, digestion solutions, and digestion stop solutions.
1. PBS Buffer Solution
Phosphate-buffered saline (PBS) is a fundamental solution used in cell factory cultures. Its primary purpose is to maintain a stable pH environment and provide an appropriate ionic balance, preventing cells from being damaged by pH fluctuations. Common uses of PBS include diluting biological agents, such as viruses, and rinsing cells to remove residual culture media. PBS’s consistent pH and salt concentration ensure the cells’ physiological state and biological activity are maintained. Compared to distilled water or saline, PBS offers the advantage of pH regulation and helps buffer against environmental shifts, which is essential for long-term cell stability.
2. Digestion Solutions
Digestion solutions are essential during cell dissociation and passaging. The most commonly used solutions include trypsin and EDTA (ethylenediaminetetraacetic acid). Trypsin works by hydrolyzing proteins in the extracellular matrix, facilitating the separation of cells or tissue fragments. EDTA, on the other hand, chelates calcium ions, reducing cell-to-cell adhesion. The use of these digestion solutions must be carefully controlled in terms of concentration, temperature, and pH, to avoid over-digestion, which can negatively impact cell viability and function.
3. Digestion Stop Solutions
Digestion stop solutions are used to halt the digestion process, typically containing high concentrations of serum or specific enzyme inhibitors. Their main function is to neutralize the activity of the digestion enzymes, preventing further cell breakdown and preserving cell integrity. Proper use of stop solutions is critical to ensuring high cell viability and the accuracy of experimental results.
Conclusion
In the cell factory analogy for large-scale cell culture, the correct application of PBS buffer, digestion solutions, and digestion stop solutions is crucial for maintaining cell health and improving productivity. By carefully selecting and using these solutions, researchers can ensure successful cell culture processes and high-quality biological products.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.