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When culturing cells, serum is an essential nutrient that increases adhesion factors, growth factors, binding proteins, etc. for cell growth. When using serum, we will involve the dispensing operation of serum, so how should it be dispensed into PETG media bottles?
1. Thawing
Take the serum out of the refrigerator at -20 degrees, thaw at room temperature (or immerse in tap water) (it takes about 30 minutes to 2 hours, or put it in a refrigerator at 4 degrees overnight; if it is not inactivated immediately after thawing, you can put it in 4 Store temporarily in the refrigerator).
2. Inactivation
Water bath, 56°C, 30 minutes, and shake evenly at any time. Take it out, put it on ice immediately, and let it cool down to room temperature naturally (about 1-3 hours). During the heat inactivation process, proper shaking at regular intervals can reduce the occurrence of precipitation.
125ml Square PETG Media Bottles
3. Packing
Transfer to the aseptic room, put the serum into 50-100ml PETG media bottle in the ultra-clean bench, seal it, and store it at -20°C for later use. Pay attention when dispensing: shake the serum gently for several weeks in advance and mix well; pay attention when blowing out the serum with a pipette: do not blow out air bubbles, the serum is very viscous and it is easy to produce air bubbles. If bubbles form, run over the flame of an alcohol lamp.
The above are the specific operation steps for serum dispensing. Be careful not to put your hands above the open bottle mouth. The dispensing speed should be fast to avoid the sedimentation bacteria falling into the bottle mouth of the PETG media bottles.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.