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Serum, as an excellent aid in cell culture, plays a significant role in the growth process of cells. The quality of serum directly affects the effectiveness of cell culture, and excessive precipitation can be a cause for concern. So, how can we prevent precipitation in PETG serum bottles?
Firstly, it's important to have a correct understanding of precipitation. When serum is thawed, the appearance of turbidity and flocculent substances should not cause alarm; this is a normal phenomenon. Common precipitates include fibrinogen, calcium phosphate, cholesterol, fatty acid esters, and some proteins. The amount of serum precipitation does not directly correlate with serum quality. If serum precipitation occurs, there's no need to worry; typically, centrifuge at 400-600g for 5 minutes, remove large particle precipitates, and use the supernatant.
Of course, if you still feel uneasy, you can take the following measures to minimize the occurrence of precipitation in PETG serum bottles:
Correct Thawing: Thaw at 2℃~8℃, intermittently and gently shaking during the thawing process. Thawing at room temperature or in a water bath can increase precipitation.
Proper Use and Storage: Avoid unnecessary heat inactivation (which can cause partial protein denaturation and precipitation, affecting serum quality). Serum and complete culture media usually only need to be preheated at room temperature, avoiding preheating at 37℃. Avoid repeated freezing and thawing, UV exposure, and long-term storage at 2-8℃. After aliquoting, store at -20℃ for long-term storage and dissolve as needed.
Above, we have discussed the types of precipitates in PETG serum bottles and methods to avoid their occurrence. In conclusion, precipitation in serum does not necessarily affect cell growth; mastering the correct thawing and storage methods is key.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.