English
In cell culture experiments, the quality of cell culture flasks directly affects cell growth and the accuracy of experimental results. Among them, pyrogenic contamination is an often overlooked but very harmful problem. Pyrogens mainly come from bacterial metabolites and their endotoxins, especially Gram-negative bacteria have the strongest pyrogenic activity. If cell culture flasks are not treated effectively, pyrogens can inhibit cell growth and even lead to experimental failure.
Dangers of pyrogen contamination
Pyrogens affect cell culture in the following ways:
Inhibiting cell growth: interfering with cell metabolism, leading to slow growth or death.
Affecting experimental results: contamination may lead to biased data, affecting research conclusions.
Increasing the cost of experiments: failure of experiments can result in a waste of time and resources.
How to avoid pyrogenic contamination?
Aseptic production and handling
Cell culture flasks should be produced in a high clean room to avoid microbial contamination.
Choose certified products
Give preference to cell culture flasks certified by ISO or other authoritative organisations to ensure that they are free of pyrogens.
Choose well-known brands to ensure product quality and consistency.
Regular Replacement and Maintenance
Avoid using the same batch of culture flasks for a long time to prevent surface abrasion or chemical residues.
Regularly check the status of culture flasks and replace them when broken or contaminated.
Pyrogenic contamination is a hidden threat in cell culture, but it can be effectively avoided by choosing high-quality cell culture flasks and following standard practices. Pyrogen-free cell culture flasks provide a safe environment for cell growth and guarantee the accuracy of experimental results.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.