English
Cell factory are indispensable facilities in modern biotechnology, used for the large-scale cultivation and production of cells. During the cultivation process, cell passage is a crucial step in maintaining cell growth and proliferation, and the precision of this operation directly impacts product quality and yield.
Preparation Before performing the cell passage, it is essential to prepare an adequate amount of cell culture. This involves taking the culture from previous containers and carefully checking the condition and density of the cells. Ensuring that the cells are in a healthy state and growing well is critical to the success of the passage process.
Pre-treating Culture Containers Select appropriately sized cell factory vessels and add a suitable amount of culture medium or solution based on the anticipated cell proliferation. This step ensures that the cells have sufficient nutrients and space to continue growing healthily during the passage process.
Washing and Detaching Cells The cells are first washed using sterile buffer solutions such as PBS or sterile culture medium to clean them from their original culture environment. Afterward, suitable cell dissociation enzymes or mild buffer solutions are used to dissociate the cells into single-cell suspensions, which is necessary for subsequent counting and passage operations.
Cell Counting and Seeding Using specialized cell counting instruments or manual counters, the dissociated cells are accurately counted. This step is crucial for determining the concentration and number of cells, allowing for the calculation and adjustment of the passage ratio. The appropriate amount of cells is then seeded into the pre-treated cell factory containers according to the set ratio to continue their growth.
Importance of Sterile Techniques It is essential to strictly follow sterile practices throughout the entire passage process. Cells are highly susceptible to contamination from external microorganisms, and any improper handling can lead to the failure of the culture or a decline in quality. Therefore, it is recommended to use dedicated tubing systems for cell factories to reduce the risk of contamination, ensuring the purity of the cells and the sterility of the culture environment.
This careful and methodical approach ensures the success of cell passage operations, supporting both cell health and high-quality bioproducts.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.