English
Ukuhlanganiswa kwamaseli kuyinkinga evamile uma usebenzisa amaflask amasiko eseli kusiko lweseli. Ukuhlangana kwamaseli kuzothinta ukukhula okuvamile nokukhiqizwa kabusha kwamaseli. Bese-ke, ixazululwa kanjani le nkinga?
Okokuqala, ukunqwabelana kwamaseli akuthinti inqubo yesiko lamaseli. Uma ingqamuzana inohlaka olucacile kanye nomzimba weseli okwazi ukungena, ohlanganiswe ngokufanayo, kufakazela ukuthi ingqamuzana likhula kahle. Kodwa-ke, ezimweni eziningi, amaseli ahlanganisiwe anezinhlaka ezingaqondakali, ukukhanya okungahambi kahle, ngisho ne-syncytia, ehambisana nemfucumfucu yamangqamuzana, okufakazela ukuthi amangqamuzana abenempilo noma anesifo futhi asesimweni esibi. Kunezindlela ezimbalwa zokuxazulula inkinga yokuhlangana kwamaseli:
1. Uma i-cell clumping ingathinti ukukhula, ayikho inkinga, kodwa kuba nzima kakhulu ukubala. Ngesikhathi sokugaya, ngaphambi kokuba amangqamuzana ashintshe asuke esimweni se-polygonal abe yindilinga, cindezela udonga oluseceleni lweflask yesiko leseli ngesandla sakho ukwenza amangqamuzana awele odongeni (kungcono ukuthi ungaqhumisi, okungalungile ukuma nokukhula kwamaseli). Ku-
2. Uma uzwa i-DNA yamaseli afile, engeza amaconsi ambalwa e-DNAse oyinyumba (1mg/ml encibilikiswe emanzini) ekumisweni kweseli ukuze uphule umucu we-DNA. Vuthela amaseli kahle ukuze uvimbele ukulimala okubonakalayo kulwelwesi lwamaseli.
3. Uma ama-cell clumps ebonakala ngeso lenyama, ake amaseli ame cishe isigamu somzuzu, bese u-pipette ingxenye engenhla yokumiswa kweseli ngaphandle kwama-clumps okudlula. Uma izigaxa zamangqamuzana zingabonakali ngeso lenyama, okwamanje ayikho indlela ekahle yokuhlukanisa, futhi lawa maseli angasuswa kancane kancane ngemva kokuba isimo seseli sesithuthukile. zingelashwa ngokwezindlela ezingenhla. Ngesikhathi senqubo yesiko lamaseli, kuyadingeka ukuthi kubhekwe isimo sokukhula kwamaseli ngesikhathi futhi wenze izinguquko ezihambisanayo ngokuya ngesimo.
If the cells in the cell culture flasks appear clumping, they can be treated according to the above methods. During the cell culture process, it is necessary to observe the growth status of the cells in time and make corresponding adjustments according to the situation.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.