Ipuleti le-cell culture liyisiko eliyinhloko elisebenzisekayo kusikompilo lwamaseli amancane. Sivamise ukuhlangana nokwenzeka kokuhlanganisa amaseli nokusabalalisa okungalingani ngesikhathi sokusetshenziswa. Usixazulula kanjani lesi simo?

Lapho uhlangabezana nokusatshalaliswa okungalingani kwamaseli kupuleti le-cell culture, qala ubheke ukuthi amaseli axutshwa kanjani, noma ngabe amapayipi noma ukunyakazisa ipuleti le-culture. Uma ingeyakamuva, futhi inyakaza kuyindilinga, kungenzeka ukuthi kungenxa yamandla amaphakathi. Amaseli aphonswa engxenyeni ye-peripheral, okuholela esikhathini esingaphansi kwamasonto amane phakathi kwamaseli. Ngaphambi kokutshala ipuleti lembewu, faka ipuleti lesiko ku-incubator amahora ambalwa wokugcwala bese ulikhipha. Lapho i-inoculating amaseli, amandla kufanele abe lula. Ungasebenzisa i-dripper ukuze wengeze kancane kancane ohlangothini lwepuleti kahle ukuze uvumele ukumiswa kweseli kugeleze emithonjeni yamapuleti, ukuze amaseli akhulisiwe akhule ngokuyisisekelo ngokulinganayo. Ungalokothi usebenzise i-oscillator ukuze i-oscillate, ngaphandle kwalokho amaseli azonqwabelana.

Uma buncane ububanzi bembobo yepuleti lesiko, yilapho kuba sobala nakakhulu ukungalingani kwamaseli. Lesi senzakalo sivame kakhulu kumapuleti angu-24-well kanye nama-96-well, ngoba udonga lwe-liquid lwenza isiko eliphakathi nendawo emthonjeni hhayi indiza ewuketshezi, futhi ukusetshenziswa kwalezi zipuleti ezimbili zemithombo kungenxa yesidingo Ngaphezu kwalokho ukungenelela kanye nezinye izizathu, akunakwenzeka ukwengeza inani elanele le-culture medium, ukuze amaseli avele "iqoqo le-edge" ne-culture medium. Uma inani lamaseli afakwe i-inoculated likhuphuka ukuze kube namaseli afanayo phakathi nendawo yomthombo, yiziphi izinkomba okufanele zibhekwe, uma kuyi-MTT, i-Immunohistochemistry izothinta imiphumela ngenxa yokwehlukaniswa kwamaseli.

Lapho ukugaya amaseli, naka pipetting ngokulinganayo ukugwema amaseli clumps, kanye nenani isiko medium emithonjeni cell isiko plate kufanele kwanele. Ngokuvamile, engeza i-culture medium eyanele ngesikhathi sokujova, futhi ushintshe okuphakathi kanye lapho wengeza ukungenelela. Inani le-intervention medium plus culture medium lilingana nokujovwa Kulesi simo, into ethi “edge collection” izothuthukiswa.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.