English
Emasikweni amaseli amakhulu, izimboni zamaseli izinto ezisetshenziswayo ezivame ukusetshenziswa, ezisetshenziselwa kakhulu isiko lamaseli abambelelayo. Ukukhula kwamangqamuzana kudinga imisoco ehlukahlukene, ngakho-ke yini?
1. Isikhungo sezamasiko
Isizinda sosikompilo samangqamuzana sinikeza amangqamuzana embonini yamaseli imisoco edingekayo ukuze akhule, okuhlanganisa amacarbohydrates, ama-amino acid, usawoti wezinto ezingaphili, amavithamini, njll. Ngokwezidingo zomsoco zamangqamuzana ahlukene, kunezinhlobonhlobo zokwenziwa. imidiya ongakhetha kuyo, njenge-EBSS, Ukhozi, i-MEM, i-RPMll640, i-DMEM, njll.
2. Ezinye izithasiselo
Ngaphezu kwemisoco eyisisekelo ehlinzekwa yimithombo yezindaba yokwenziwa ehlukahlukene, ezinye izakhi, njenge-serum nezici, zidinga ukwengezwa ngokuvumelana namaseli ahlukene kanye nezinjongo ezihlukahlukene zesiko.
I-Serum inikeza izinto ezibalulekile ezifana ne-extracellular matrix. , izici zokukhula kanye ne-transferrin, kanye ne-fetal bovine serum isetshenziswa kakhulu. Ingxenye ye-serum eyengeziwe kufanele inqunywe ngokuya ngamaseli ahlukene kanye nezinjongo ezihlukene zocwaningo. I-10% ~ 20% i-serum ingagcina ukukhula okusheshayo kanye nezinga lokusabalala kwamaseli, okubizwa ngokuthi ukukhula okuphakathi; ukuze kugcinwe ukukhula okuhamba kancane noma ukungafi kwamangqamuzana, i-serum engu-2% ~ 5% ingangezwa, ebizwa ngokuthi i-maintenance medium.
I-Glutamine iwumthombo obalulekile we-nitrogen wokukhula kwamangqamuzana futhi idlala indima ebalulekile ekukhuleni kwamangqamuzana. kanye ne-metabolism. Kodwa-ke, ngenxa yokuthi i-glutamine ayizinzile futhi yonakaliswa kalula esixazululweni, cishe u-50% we-glutamine ungabola ngemva kwezinsuku ezingu-7 ku-4°C. I-amino amide idinga ukungezwa ngaphambi kokusetshenziswa.
Ngokujwayelekile, imidiya ehlukahlukene kanye ne-serum isetshenziselwa isiko lamaseli, kodwa ukuze kuvinjwe ukungcoliswa kwamaseli ngesikhathi sosiko, inani elithile lemithi elwa namagciwane, njenge-penicillin, i-streptomycin, i-gentamicin, njll., ziphinde zengezwe kuhlelo lwamasiko.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.