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Amaseli abambelelayo njengamanje awuhlobo oluyinhloko lwesiko lamaseli. Zidinga ukukhangiswa phezu kokusekelwa futhi zithembele ezintweni ezinamathiselwe ezifihliwe ngokwazo noma zinikezwe endaweni yesiko ukuze zikhule futhi zande phezulu. Kwesinye isikhathi sithola ukuthi amaseli kokuthi ama-cell culture flasks awakwazi ukunamathela odongeni. Yini ebangela lokhu?
Ukunamathela kwamaseli kuwukusabela kokujwayela indawo ezungezile. Amaseli anokuminyana okukhulu kune-culture medium azohlala phansi. Uma amaseli ezosinda, kufanele athuthukise le ndawo, akhiphe i-matrix engaphandle kwamaseli, athuthukise ukwakheka kwendawo engaphansi, bese enamathela ngokwemvelo endaweni engaphansi. Phezu kwakho. Amaseli anesisindo esingaphansi kwamanzi azontanta endaweni ewuketshezi, ngakho-ke akunakwenzeka ukunamathela endaweni engaphansi, kodwa uma isisombululo sempucuko sigcwaliswa ukuze amaseli akwazi ukuxhumana nodonga olungaphezulu lwamaflask amasiko eseli, angakwazi. futhi unamathele odongeni. Uma amaseli enganamatheli odongeni, angahlaziywa kusukela kuzici ezilandelayo:
1. Into ebaluleke kakhulu ekudluleni kwamaseli yisikhathi sokwelashwa kwe-trypsin: amaseli ngokwawo ahlanganiswe uma engagayeki ngokwanele; uma ukwelashwa kwe-trypsin kukude kakhulu, kulula ukubangela ukulimala kwamaprotheni e-membrane yeseli, ukwenza amangqamuzana anganamatheli odongeni ngokuqinile, futhi umphumela wokubuka okuyingxenye-ntathu ngaphansi kwe-microscope awunamandla. , Futhi kubangele nokufa kwamangqamuzana ezimweni ezimbi kakhulu.
2. Ukuntuleka kwezinto zokunamathela: i-serum iqukethe izici ezikhuthaza ukunamathela, futhi ukungabi khona kwezinto ezinjalo ezikhuthaza ukunamathela ohlelweni lwe-serum-free medium kuzonciphisa kakhulu umphumela wokunamathela wamaseli.
3. Ukungcoliswa kwe-mycoplasma noma amagciwane.
4. Ukucushwa okungalungile nokugcinwa kwesixazululo sesiko, njenge-pH ye-alkaline ngokweqile kanye ne-Gln encane kakhulu.
5. Amaseli atholiwe awekho esimeni esihle, ayaguga futhi alahlekelwa ukunamathela kwawo.
6. Inani lamaseli ajovwe lincane kakhulu ukuthi lingakhiqiza i-matrix engaphandle kwamaseli.
7. Isivinini sokucubungula ngesikhathi sokuvuselela sihamba kancane kakhulu futhi inqubo yokuvuselela ayilungile.
Sekuhlanganiswe ndawonye, ukunganamatheli kwamaseli anamathelayo ikakhulukazi kungenxa yezizathu ezingenhla. Ukwengeza, ipulasitiki amaflask e-cell culture engazange iphathwe nge-TC ngaphezulu izophinde ibe nomthelela ekusebenzeni kokunamathela kwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.