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I-i-cell culture flask iwuhlobo lwezinto ezisebenzisekayo zesiko lamaseli zohlobo lwebhodlela. Ukuze kuthuthukiswe ukusebenza kokubambelela kwamaseli, izinto ezisetshenziswayo ziyambozwa lapho kutshalwa amaseli ayisisekelo. Isigcinalwazi esivame ukusetshenziswa sihlanganisa lokhu okulandelayo:
Polylysine: Iyinhlanganisela yokwenziwa, i-amino acid ene-positive, ekhuthaza ukunamathela kwamangqamuzana ngokushintsha i-electrode ebusweni be-substrate yesiko. Isetshenziswa kakhulu kusiko lwamaseli ukuze kuhlanganiswe ukukhuthaza ukunamathela kwamaseli. Ngaphezu kokukhuthaza ukunamathela kwamangqamuzana nokukhula, ukumbozwa kwe-polylysine ngesisombululo sokumboza kungakhuphula izinga lokusinda kwamangqamuzana ayinhloko futhi kukhuthaze ukuphuma kwe-neurite.
Fibronectin: I-high-molecular glycoprotein eyaqala ukucwaningwa futhi yatholakala phesheya ngo-1974 , futhi inemisebenzi ehlukahlukene yezinto eziphilayo. I-Fibronectin iyi-glycoprotein enesisindo samangqamuzana angama-440-500kDa. Itholakala kumaprotheni angaphezulu kwamaseli kanye ne-plasma. Ihlanganisa nama-cell membrane surface receptors kanye nezingxenye ze-extracellular matrix ukukhuthaza ukunamathela kwamangqamuzana nokukhula okunwetshiwe.
I-Gelatin: ingxube yesisindo samangqamuzana amaprotheni ancibilikayo emanzini atholakala ku-collagen, isisombululo se-gelatin esiwuketshezi esingu-0.2% sisetshenziselwa ukumboza imikhumbi yesiko. ukukhuthaza ukunamathela kwamaseli. Yethula ama-stem cells omuntu ukuze ahlukaniseke, amaseli athile ayisisekelo kanye namaseli angafi.
Okungenhla kukhona isivimbeli sokumboza esivame ukusetshenziswa kumaflask e-cell culture. Ukukhetha okukhethekile kwe-coating buffer kuncike ohlotsheni nezici zamaseli. Kodwa-ke, kufanele kuqashelwe ukuthi isikhawu phakathi kokumboza nokusetshenziswa kwebhodlela akufanele sidlule usuku olulodwa. Uma isikhathi eside isitsha esiboshwe sigcinwa, ukusebenza kwayo kokunamathela kuzothinteka.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.