Lapho sihlakulela amangqamuzana, sivame ukukhathazwa ukungcoliswa kwamangqamuzana okuhlukahlukene. Uma amaseli engcolisiwe, ngeke nje athinte ukukhula kwamaseli, kodwa kwezinye izimo, kufanele silahle amaseli futhi siwahlakulele kabusha, okuzothinta inqubo yokuhlola. Amafekthri Amaseli asetshenziswa kakhulu kusikompilo lwamaseli amakhulu, futhi ukungcoliswa kwawo okuvamile ngokuyinhloko kuwela ezigabeni ezintathu ezilandelayo:
1. Ukungcoliswa ngokomzimba: ikakhulukazi kubhekiselwa emonakalweni wamaseli ngezinto ezibonakalayo ezifana nezinga lokushisa, ukudlidliza, imisebe, nemisebe. Njengokuchayeka kwe-cell culture medium kuya emisebeni noma ekukhanyeni kwe-ultraviolet, kuzodala izinguquko kumetabolism yamangqamuzana. Kunamathuluzi azungeze i-incubator angakwazi ukukhiqiza ukudlidliza kwemishini, okungase futhi kube nomthelela othile ekukhuleni kwamaseli.
2. Ukungcoliswa kwamakhemikhali
Isisetshenziswa namanzi asetshenziselwa isiko lamaseli kufanele kubulawe amagciwane ku-autoclave. Phakathi kwazo, i-serum iyindlela evame ukusetshenziswa esikweni lamaseli, futhi i-serum inokungcoliswa kwamakhemikhali okungaba khona. Ukwengeza, ukwakheka kwe-serum akuqinisekile, futhi imiphumela ye-serum ekukhuleni kwamaseli ahlukene, okuhlanganisa ne-toxic and side effects, ihlukile.
3. I-Microbial contamination
I-Microbial contamination ibhekisela emithonjeni ehlukahlukene yokungcola njengama-bacteria, isikhunta, i-mycoplasmas, nezikelemu ezimnyama ezikhula lapho amangqamuzana ekhula ezimbonini zamaseli. Amachashazi amancane amnyama avela kumaseli ngemva kokungcoliswa izimbungulu zeglue ezimnyama, futhi ukunyakaza okungavamile kwakungabonwa ngaphansi kwesibonakhulu sokukhulisa kakhulu. Imfucumfucu yendawo yesiko ayibonakali, futhi ayinawo umthelela omkhulu esimweni sokukhula kwamaseli.
Lokhu okungenhla kuyizinhlobo ezintathu ezivamile zokungcola lapho kusetshenziswa izimboni zamaseli kumaseli amasiko. Ngaphandle kokuthintwa yimithombo yokungcola, amaseli anezidingo eziqinile ngendawo yokukhiqiza, izinga lokushisa, ingcindezi ye-osmotic, inani le-pH nezinye izici. Indawo efanele yokukhula kuphela engaqinisekisa ukukhula okuhle kwamaseli. isimo.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.