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Ngokuthuthuka okusheshayo kwezimboni ezifana ne-biopharmaceuticals, imithi yokugoma, namasosha omzimba e-monoclonal, ukubaluleka kokuthi amafekthri amaseli kusikompilo lwamaseli wezinga elikhulu kuye kwagqama kakhulu. Ukwenziwa kwamaseli kuwumsebenzi onzima, onokulawula okuqinile kwendawo yokukhula kwamaseli, ukwengezwa kwemithombo yezindaba yamasiko, kanye nokuvunwa kwamaseli.
Lapho usebenzisa imboni yamaseli ukuvuna amaseli, lezi zinqubo ezilandelayo kufanele zibe kulandelwa ngokuqinile:
1. Thela ikhambi le-culture ngemva kokufukamela, futhi ugeze nge-calcium-free and magnesium-free phosphate buffer (CMF-PBS) (40-50 ml/ungqimba), futhi uphinde ukuwasha uma kunesidingo.
2. Ukugaya: Isixazululo sokugaya (10-40 ml/ungqimba) sishisisa kusengaphambili.
3. Ukuqoqwa: I-Centrifuge ku-1000 rpm imizuzu emi-5 ukuze ikhiphe isisombululo sokugaya futhi iqoqe amaseli.
4. Ukugeza: Geza incubator egayiwe nge-CMF-PBS noma isiko.
Naka amaphuzu alandelayo lapho uvuna amaseli:
1. Qinisekisa ukuthi i-CMF-PBS icwiliswe ngokuphelele endaweni ngayinye yesiko, futhi unyakazise kahle ifekthri yeseli emuva naphambili ukuze uhlanze uketshezi lwamasiko olusele.
2. Isixazululo sokugaya sisatshalaliswa ngokulinganayo kungqimba ngalunye. Tshekisa i-incubator ngobumnene emuva naphambili ukuze uqinisekise ukuthi isisombululo sokugaya sicwiliswa ngokuphelele endaweni yesiko. Thepha ngobumnene i-incubator ukuze usize amaseli ahlukane phezulu.
3. Embonini yeseli, njengoba isimo sokugayeka kokudla kwamaseli ongqimba oluphakathi asikwazi ukubonwa ngokucacile, kunconywa ukuthi ubhekisele esimweni sokugaya seflask yesiko noma i-monolayer cell factory ngaphansi kwezimo ezifanayo zesiko. Ungasebenzisa futhi inkundla yokubuka isiko lamaseli ezendlalelo eziningi ukuze ubone isimo sokukhula sengqimba ngayinye yamaseli.
3. Uma kunenombolo enkulu yamaseli esixazululweni sokuhlanza noma ungqimba lwesiko lwefekthri yeseli, kuyadingeka ukuhlanza izikhathi eziningi noma ukulungisa ukusebenza kokugaya kwamaseli.
4. Ukuchezuka kancane kwezinga lokushisa lesiko kungase kuthinte izinga lokuvuna lamaseli, ngakho-ke bhekisisa izinga lokushisa lesiko yizinga lokushisa elimisiwe.
Okungenhla kuyinqubo nezinyathelo zokuphepha zokuvuna amaseli ku-fektri yamaseli. Amaseli azwela kakhulu emvelweni. Ukwenza umsebenzi omuhle ekuvuneni amaseli kuyisihluthulelo sempumelelo yesiko lamaseli, futhi zonke izixhumanisi zidinga ukulawulwa ngokuqinile.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.