English
Ubuchwepheshe be-cell Culture ubuchwepheshe obusetshenziswa kakhulu emikhakheni ye-biopharmaceuticals, amasosha omzimba e-monoclonal, kanye nokwelashwa kwamaseli. Amafekthri Amaseli zisetshenziselwa ikakhulukazi isiko lamaseli amakhulu. Ukukhula kwamangqamuzana kuthintwa izici eziningi, izinga lokushisa eliyingxenye ebaluleke kakhulu.
Ngaphansi kwezimo ezijwayelekile, izinga lokushisa elifanele le-in vitro culture yamangqamuzana ezilwane ezincelisayo kanye nezinyoni zezinyoni lingu-37-38 °C. Ukushisa okuphezulu kakhulu noma okuphansi kakhulu kuzothinta ukukhula kwamaseli. Ikhono lamaseli ukubekezelela izinga lokushisa eliphansi linamandla kunokumelana nokushisa. Emazingeni okushisa aphansi, umsebenzi we-metabolic kanye nokuhlukaniswa kwamaseli enuzi kuyancishiswa. Lapho izinga lokushisa lingekho ngaphansi kuka-0 ° C, nakuba lithinta i-cell metabolism, alinawo umphumela olimazayo; lapho amaseli ebekwe ku-25 kuya ku-35 ° C, amangqamuzana asengakwazi ukusinda futhi akhule, kodwa ijubane liyancipha; Emuva ku-37 ℃ amaseli empucuko angaqhubeka nokukhula.
Izinga lokushisa eliphezulu alilungile kusiko lwamaseli. Amaseli akhuliswa ku-39-40 ° C ngehora elingu-1, angalimala ngezinga elithile, kodwa angakwazi ukubuyisela, kodwa awakwazi ukubekezelela ukunyuka kwezinga lokushisa kwe-2 ° C amahora ambalwa, okungukuthi, uma ekhuliswa. ku-41-42 ° C ngehora elingu-1, umonakalo weseli unzima. Iningi lamaseli abulawa lapho izinga lokushisa lingaphezu kuka-43°C. Ukushisa okuphezulu kubangela ukungasebenzi kwama-enzyme, ukucekelwa phansi kwe-lipids, ukucekelwa phansi kwe-nuclear division, ukukhiqizwa kwe-coagulase, ukuhlangana kwamaseli, kanye nokuguqulwa kwamaprotheni. Ngakho-ke, imboni yamaseli kufanele igcine izinga lokushisa elifanele futhi igweme izinga lokushisa eliphezulu lapho ihlakulela amaseli. Ezinye zihlanganisa ingcindezi ye-osmotic, indawo eyinyumba, igesi, ne-pH.
In conclusion, cell growth in a cell factory is affected by multiple factors, and temperature is only one of them. Others include osmotic pressure, sterile environment, gas, and pH.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.