English
Lapho uhlakulela amaseli ahlukahlukene, amaflask e-cell culture wuhlobo lwezinto ezisetshenziswayo zesiko lamaseli ezisetshenziswa kakhulu. Asetshenziselwa kakhulu isiko lamaseli anamathelayo, futhi imininingwane isukela ku-25ml kuya ku-225ml. I-hamster kidney culture medium izosebenzisa lokhu okusebenzisekayo, futhi izinyathelo ezithile zesiko layo zimi kanje:
1. Umsebenzi wokulungiselela wesiko lamaseli:
1) Lungiselela i-MEM medium; izinga eliphezulu le-fetus bovine serum, 10%; amasosha omzimba aphindwe kabili 1%.
2) Izimo zesiko: Isigaba segesi: umoya, 95%; i-carbon dioxide, 5%. Izinga lokushisa: 37 degrees Celsius, umswakama we-incubator ongu-70%-80%.
3) Isixazululo sokubanda: 90% serum, 10% DMSO, isilungele ukusetshenziswa.
2. Ukucutshungulwa kweseli:
1) Ukutholwa kwamaseli a-cryopreserved: Shesha unyakazisa futhi uncibilikise ishubhu e-cryopreserved equkethe u-1 mL weseli ukumiswa kubhavu wamanzi ongu-37°C, wengeze kushubhu ye-centrifuge equkethe u-4-6 mL we-medium ephelele bese uxuba. kahle. I-Centrifuge ku-1000RPM ye-3-5min, lahla amandla amakhulu, futhi umise kabusha amaseli endaweni ephelele. Ukumiswa kweseli kwabe seyengezwa kuflask yesiko leseli eliqukethe u-6-8 ml we-medium ephelele futhi ekhuliswe ku-37 ° C ngobusuku bonke. Ukukhula kwamangqamuzana nokuminyana kwamangqamuzana kwaqashelwa ngesibonakhulu ngakusasa.
2) Indima yeseli: Uma ukuminyana kwamaseli kufinyelela ku-80% -90%, kungenziwa ngendlela engafani.
3) Ukulondolozwa kwamaseli: Ngemva kokuthola amaseli, kutuswa ukuba kufrize iqoqo lezimbewu zamaseli ezindimeni zokuqala ezi-3 zesiko ukuze kuhlolwe okulandelayo.
Lezi ngenhla ziyizinyathelo eziqondile zokukhuliswa kwamaseli ezinso ze-hamster kumaflask e-cell culture. Ngaphezu kwalokho, lapho amaseli e-cryopreserved, qoqa amangqamuzana agaywe uwafake eshubhuni eliyi-centrifuge ngokwenqubo ye-cell passage, bese usebenzisa i-hemocytometer ukuze uwabale ukuze unqume ukuminyana kwe-cryopreservation yamaseli. Ukuminyana okuqandisiwe okunconyiwe kwamaseli ajwayelekile ngu-1×106~1×107 amaseli asebenzayo/ml.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.