English
I- flask cell culture iyisiko leseli elikhethekile elisebenzisekayo elinomklamo oyisikwele nobubanzi bebhodlela. Ngokuya ngezidingo ezihlukene zokusetshenziswa, lokhu okusebenzisekayo kuhlukaniswe ngo-25cm2, 75cm2, 175cm2, 225cm2 nokunye ukucaciswa ukuze kuhlangatshezwane nezidingo ezihlukene zabahloli.
Usayizi omncane: Usayizi weseli ubhekise ku indawo yesiko yeflask yesiko lamaseli. Amabhodlela angu-25cm2 kanye ne-75cm2 asetshenziselwa kakhulu ukubuyisela amaseli kanye nokwandisa kancane. Ukuvuselela amaseli yigama lebhayoloji elisho inqubo yokutshala kabusha amaseli aqandiswe ku-nitrogen ewuketshezi noma esiqandisini esingu-70°C ukuze kubuyiselwe ukukhula. Ukunwetshwa kweseli kubhekise ekwandiseni nasekukhuliseni kabusha amaseli akhona.
Usayizi omkhulu: 175cm2 kanye no-225cm afanele kakhulu isiko lamaseli esikali esimaphakathi kanye nokuvezwa kwamaprotheni e-eukaryotic. Uma isetshenziselwa isiko lamaseli, ngokuvamile ibhekisela kumaseli abambelelayo, futhi amanye amaseli amisiwe nawo asebenzisa lokhu okusebenzisekayo. I-Eukaryotic protein expression iyindlela evame ukusetshenziswa yokuveza amaprotheni ahlanganayo eminyakeni yamuva nje. Yengeza eminye imisebenzi engekho ezinhlelweni zokuvezwa kwamaprotheni e-prokaryotic. Isibonelo, ingakha amabhondi e-disulfide azinzile phakathi nenkulumo ye-eukaryotic. Guqula kahle iphrotheni ukuze wenze iphrotheni eveziwe ibe umsebenzi wemvelo kakhudlwana esikhundleni sokucekelwa phansi noma ukwakha imizimba ehlanganisayo; ukusetshenziswa kwesistimu ye-eukaryotic expression kungenza isisho esisebenza kahle kakhulu, sisheshise inqubo yocwaningo lofuzo lwabantu kanye nocwaningo lwezidakamizwa.
Okungenhla ukusetshenziswa kuka-amaflask e-cell culture kokucaciswa okuhlukile. Kungakhathaliseki ukuthi yisiphi isici esisetshenziswayo, izimfuneko eziyisisekelo ezifana ne-DNase-free, i-RNase-free, ne-pyrogen-free ziyafana, okuyisidingo esiyisisekelo sokukhula kwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.