English
Iningi lamaseli akhuliswe ku-vitro angamaseli anamathelayo. Ukukhula kwamaseli anjalo kufanele kube nendawo yokusekela engaxhunywa kuyo. Amaseli angakhula futhi aphindaphindeke phezulu ngokuthembela ezintweni ezinamathiselwe ezifihliwe ngokwazo noma ezihlinzekwe endaweni yamasiko. Ngakho-ke, yiziphi izidingo zezinto ezidliwayo zesiko leseli lamaseli abambelelayo?
Amaseli ancike e-anchorage kufanele axhunywe odongeni lomkhumbi wamasiko (ibhodlela) lapho ekhuliswa. Uma amaseli enamathiselwe, asakazeka ngokushesha, bese eqala i-mitosis, futhi asheshe angene esigabeni sokukhula kwe-logarithmic. Ngemuva kwezinsuku ezimbalwa, amangqamuzana azovala ingaphezulu lomkhumbi wamasiko futhi enze i-monolayer yeseli eliminyene. Izinto ezisetshenziswa ngamaseli asetshenziswayo kumaseli abambelelayo zihlanganisa amafekthri amaseli, amapuleti e-cell culture, ama-cell spinner flasks, njll. Okwamanje, imikhiqizo eminingi esetshenziswa amaseli emakethe ngokuyinhloko iyizinto ze-polymer ezifana ne-PS, i-PC, ne-PE. Lolu hlobo lwezinto ezibonakalayo alunazo izici zengilazi ze-hydrophilic, futhi indawo engaphezulu idinga ukuphathwa ngokukhethekile ngaphambi kokuba isetshenziselwe ukutshala amaseli anamathelayo. Izinto ezisetshenziswa ngokukhethekile ekusetshenzisweni kwe-cell culture zineqembu le-hydrophilic elethulwe phezulu, elivumela amaseli ukuthi abambelele kangcono endaweni esetshenziswayo, ekhuthaza ukukhula kwamangqamuzana nokuzala.
Ukukhula kweseli kudinga indawo ethile, njengekhabhoni. i-dioksidi, i-pH, ubunyumba, ingcindezi ye-osmotic, njll., ngakho-ke izinto ezisetshenziswayo ze-cellculture kumele zifakwe inzalo futhi zifinyelele izimo ezingenayo i-DNase kanye ne-RNase ngaphambi kokuthi zisetshenziswe, ukuze kugwenywe ukungcoliswa kwamaseli okuthinta inqubekelaphambili yesiko lamaseli.
Ukwelashwa nokuvalwa kwe-Surface TC yizimfuneko ezimbili eziyisisekelo zezinto ezisetshenziswayo ze-cell culture kumaseli abambelelayo, futhi ziyizimfuneko zenqubekelaphambili eshelelayo yomsebenzi wamaseli. Ngakho-ke, udinga ukuqaphela kakhulu lapho ukhetha imikhiqizo esebenzisekayo.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.