English
I-i-cell culture flask iwuhlobo lweseli olusebenzisekayo olusetshenziswa ocwaningweni lwempilo nasekuzijwayezeni. Isetshenziselwa kakhulu ukutshalwa kwamaseli anamathelayo. Ngesikhathi sokusetshenziswa, indlela yokusebenza efanele iyisidingo sokuqinisekisa ukuqhubeka kahle kokuhlolwa. Naka lokhu okulandelayo uma usebenzisa lokhu okusebenzisekayo Yisikhathi sini:
1. Mayelana nesivalo: Ungaqinisi isivalo seflask yamasiko eseli alahlwayo kakhulu ukuze uqinisekise ukushintshaniswa kwegesi. I-HEPES iyibhafa engeyona i-ionic enamandla okubhafa okuhle kububanzi be-pH obungu-7.2-7.4, kodwa ibiza kakhulu futhi ingase ibe ubuthi kwamanye amaseli ekugxilweni okuphezulu. I-HEPES buffer ingasetshenziswa ngezinga eliphansi le-sodium carbonate (0.34g/L) ukuze kuncishiswe ukwanda komfutho we-osmotic obangelwa ukwengezwa kwe-HEPES. Ngaphansi kwalesi simo sesiko, isivalo seflask ye-cellculture cell elahlayo kufanele siqiniswe ukuze kuvinjwe inani elincane le-carbonate esixazululweni sesiko ukuthi lingahlakazeki emoyeni. Uketshezi oluningi lwe-culture luqukethe i-phenol ebomvu njengenkomba ye-pH, uketshezi lwesiko eline-asidi luphuzi ngokusawolintshi, futhi uketshezi lwe-alkaline lubomvu ngokumnyama.
2. Nciphisa "umgodi wokukhula": Abasebenzisi abaningi abasebenzisi amaflask alahlwayo eseli agqitshwe ngaphambili, kodwa amaflask ajwayelekile alahlwayo. Indawo engaphezulu ayihambisani ne-mesenchymal stem cell paste endaweni yesiko elingenayo i-serum. udonga. Yibeke ku-37 ° C imizuzu engu-5-10 ukuze uhlanganise kangcono izinto ezikhuthaza ukunamathela endaweni yesiko nephansi le-flask yesiko lamaseli alahlwayo, okuthuthukisa ikhono lokunamathela lamaseli e-mesenchymal stem futhi kunciphisa amathuba "ezimbobo zokukhula. ".
3. Ukugaya amaseli: Beka ibhodlela liqonde bese uncela isiko. Uma kunamaseli amaningi akhishwe esikhumbeni se-culture medium, kusho ukuthi amaseli manje alula ukuwa. Vele ungeze u-1ml we-PBS+EDTA (0.02%) bese unyakazisa ibhodlela le-culture emuva naphambili kancane kuze kube yilapho Amaseli ewa ngokuphelele, engeza u-10ml MEM, uhlanganise kahle, ungafaki i-pipette, uhlukanise amabhodlela angu-2, uma amaseli ewa ngaphandle kokukhula ngokweqile, vele wengeze i-5ml MEM, akukho ukupakisha okuhlukile. Uma amaseli ebhodleleni enamathela ngokuqinile, i-medium supernatant ayinayo imfucumfucu yokuchithwa kwamaseli, futhi amaseli akhule ngaphezu kuka-80%, afisa okuphakathi, engeza u-5ml we-PBS+EDTA (0.02%), anyakazise ngokushesha kancane, futhi enze isiko imile Gcoba ibhodlela, engeza u-1ml ka-0.05% we-pancreatin, ugaye ku-37°C isikhathi esingeqile kwemizuzu emi-3, futhi amaseli azogaywa ngokuphelele futhi ahlutshiwe. Khipha bese wengeza u-10ml we-culture medium kuya ku-pipet kancane futhi uhlanganise, futhi uhlukanise amabhodlela angu-2.
Amaseli azwela kakhulu endaweni yokukhula. Njengokusetshenziswa okuvame ukusetshenziswa kakhulu, i-amaflask e-cell culture kufanele isetshenziswe ngokucophelela futhi ilandele ngokuqinile izinqubo ezinqunyiwe ukuze kugwenywe ukusebenza okungalungile okuthinta ukukhula kwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.