English
Amafekthri Amaseliziwuhlobo lwezinto ezisetshenziswayo ezisetshenziswa kakhulu kusiko lweseli elikhulu. Amaseli ayizinto eziphilayo ezintekenteke kakhulu. Isiko le-in vitro lamaseli lidinga indawo ethile, lapho ubunyumba buyimfuneko eyisisekelo.
In vivo, isistimu yokukhipha ubuthi kanye namasosha omzimba angakwazi ukumelana nokuhlasela kwamagciwane noma ezinye izinto eziyingozi, kodwa inqubo ye-in vitro isiko, amaseli aswele ukuvikelwa kwamasosha omzimba futhi alahlekelwe amandla okuzivikela kuma-microorganisms futhi akhiphe izinto eziyingozi. Ukuqinisekisa ukuthi amangqamuzana angakhula futhi akhiqize kabusha ku-vitro, kuyadingeka ukuqinisekisa indawo yokusebenza eyinyumba, inhlanzeko yomuntu siqu, ama-reagents oyinyumba kanye nemidiya, kanye nokuphathwa kwe-aseptic. isikhunta. I-Mycoplasma ayinabo ubuthi obubulalayo, ingahlala namaseli isikhathi eside, futhi inemiphumela engaba khona kumaseli, kodwa incane ngosayizi futhi kunzima ukuyibona. Ingatholwa ngokubomvu kwe-lichen noma i-Hoechst33342 staining. Amagciwane anda ngokushesha, angaphindaphindeka ngenani elikhulu ngesikhathi esifushane, futhi akhiqize ubuthi bokubulala amangqamuzana. Kunezinhlobo eziningi zesikhunta, ezibonakala ngeso lenyama, zintanta ebusweni bendawo yesiko, futhi zingaba filamentous, tubular noma dendritic.
Uma ukungcola kwamagciwane kwenzeka kumaseli embonini yeseli, umthombo Ukungcola kufanele kuhlaziywe ngokuya ngesimo esithile, bese ukwelashwa kufanele kwenziwe ngokuya ngesimo sokungcola. Uma ukungcola kubi kakhulu, kunconywa ukuthi ulahle isiko futhi uhlakulele kabusha.
If bacterial contamination occurs in the cells in the cell factory, the source of contamination should be analyzed according to the specific situation, and then the treatment should be carried out according to the contamination situation. If the contamination is very serious, it is recommended to discard the culture and re-cultivate it.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.