English
Amagciwane e-Coliform abhekisela eqenjini lamabhaktheriya angenayo i-gram-negative angakwazi ukuvundisa i-lactose, akhiqize i-asidi negesi, i-aerobic ne-facultative anaerobic. Ekusetshenzisweni okungokoqobo, kuvame ukusetshenziswa njengenkomba yokuthi ukudla kungcoliswe indle. Phakathi kwazo zonke izindlela zokuthola, ukusetshenziswa kwe-ama-cell culture flasks ukuze kutholwe iyona esetshenziswa kakhulu. Izinyathelo zokusebenza zimi kanje:
1. Isampula kanye ne-dilution
(1) Ngokwendlela ye-aseptic, faka u-25g (noma 25mL) wesampula yokuhlola kuflask yeseli equkethe amanzi angenalutho angama-225mL (inombolo efanelekile yobuhlalu bengilazi esethiwe eflaskini) noma udaka olufakwe inzalo, futhi uyinyakazise kahle. Noma gaya ukuze wenze ukuhlanjululwa okufanayo kwe-1:10. Kungcono kakhulu ukusebenzisa i-homogenizer eyinyumba kumasampula aqinile kanye ne-centrifuge ngesivinini esingu-8000～10000r/min iminithi elingu-1 ukwenza i-dilution engu-1:10.
(2) Sebenzisa utshani obuyinyumba obuyi-1mL ukuncela i-1Ml ye-1:10 dilution, ithele eshubhuni lokuhlola eliqukethe u-9mL wamanzi oyinyumba, xukuza futhi uhlanganise ukwenza i-dilution engu-1:100, buyisela utshani obuyinyumba obuyi-1mL, cindezela Le misebenzi engenhla yenziwa ngokulandelana izikhathi eziyi-10 zokuhlanjululwa kwe-serial. .
(3) Ngokuvumelana nezidingo zenhlanzeko yokudla noma isilinganiso sokungcoliswa kwamasampula okuhlola, khetha ukuhlanjululwa okuthathu bese ujova amashubhu angu-3 ekuhlambululeni ngakunye. Ingaphinda ijovwe ngesampula ngqo.
2. Ukuhlolwa kokuqala kokuvutshelwa kwe-lactose
Lokhu kuvame ukubizwa ngokuthi ukuhlola okucatshangelwayo. Inhloso yawo ukuhlola isampula ukuze kutholakale amagciwane abilisa i-lactose ukuze akhiqize igesi. Isampula okufanele ihlolwe ifakwe kushubhu yokuvutshelwa kukasawoti we-lactose bile, ishubhu yokuvutshelwa kukasawoti we-lactose kabili isetshenziselwa inoculum engaphezu kwe-1 mL; ishubhu eyodwa yokuvutshelwa kukasawoti we-lactose isetshenziswa ku-1 mL nangaphansi kuka-1 mL. Faka amashubhu angu-3 ku-dilution ngayinye bese uwabeka ku-incubator engu-36±1℃ amahora angu-24±2. Uma wonke amashubhu okuvutshelwa kukasawoti e-lactose engakhiqizi igesi, kungabikwa njengokungemuhle kumagciwane e-coliform. Uma kukhona okukhiqizwa igesi, landela inqubo engezansi.
3. Isiko lokuhlukanisa
Amashubhu okuvutshelwa akhiqiza igesi ahlutshiwe futhi ajovwa kumapuleti e-agar aluhlaza okwesibhakabhaka i-eosin methylene, futhi akhuliswa ku-incubator engu-36±1°C amahora angu-18-24. Ngemva kwalokho, i-colony morphology yaqashelwa futhi kwenziwa ukungcoliswa kweGram, ukuhlolwa kwe-microscopic kanye nokuhlolwa kokuvutshelwa kabusha.
4. Isivivinyo sokuvutshelwa kwe-Lactose kabili
Lokho okubizwa ngokuthi ukuhlolwa kokuqinisekisa, okunhloso yayo iwukufakazela ukuthi amagciwane angenayo i-bacillus e-gram-negative ahlukanisiwe neshubhu lokuhlola elabe lihle ekuhlolweni kokuqala kokuvutshelwa kwe-lactose angakwazi ngempela ukuvubela i-lactose ukuze akhiqize igesi. Endabeni ekhethiwe ye-EMB eshiwo ngenhla, khetha i-coliform colonies engu-1 kuya ku-2 esolisayo ukuze kufakwe iGram staining, bese ujova amashubhu okuvutshelwa kwe-lactose ngesikhathi esifanayo, futhi uwabeke ku-incubator engu-36±l°C amahora angu-24±2 ukuze ubuke igesi. ukukhiqizwa. . Lapho ithubhu le-lactose fermentation likhiqiza igesi futhi ibala legram libi, amagciwane angenayo i-Bacillus kubikwa ukuthi ayakha ama-coliform; lapho ishubhu le-lactose fermentation lingakhiqizi igesi noma ibala legram liphozithivu, kubikwa ukuthi kunegethivu kuma-coliforms.
Okungenhla kuyindlela yokusebenza yokusebenzisa amaflaski amasiko weseli ukuze kutholwe izinkomba zeqembu le-E. coli. I-Escherichia coli iyinkomba ebalulekile ebonisa ukuthi ukudla kungcolile yini. Ukuba ingcweti kwendlela yokuthola kuyikhono eliyisisekelo wonke umhloli wokudla okufanele abe nalo.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.