English
I-cell passage ibhekisela enqubweni yokuhlukanisa isiko libe izingxenye ezincane, ukutshala kabusha kwesinye isitsha sosiko, bese kutshalwa. Amaflask osiko lwamaseli kukhona okusebenzisekayo okuvamile enqubweni yokudlula, ngakho-ke yiziphi izinyathelo ezithile zokudlula kweseli?
1. Khipha ama-cell culture flasks ku-incubator, fafaza u-75% wotshwala phezu kwebhodlela ngekani lesifutho sotshwala, bese ulidlulisela etafuleni elihlanzeke kakhulu.
2. Vula isivalo, ulangazelele ngobumnene isiko elidala, engeza inani elifanele lebhafa ye-PBS ukuze ugeze izikhathi ezingu-1-2 nge-Pasteur pipette, futhi ulahle isigcinalwazi se-PBS.
3. Sebenzisa i-pipette ukwengeza u-1-2ml we-pancreatin bese unyakazisa "isiphambano" ukwenza i-pancreatin ithinte amaseli ngokugcwele.
4. Dlulisela i-cell culture flask ene-trypsin esigabeni esihlanekezelwe sesibonakhulu bese ubheka ukugayeka kweseli ngaphansi kwesibonakhulu. Lapho amaseli eba yindilinga futhi ewa ngobuningi, lungiselela ukunqamula ukugaya. Fafaza ingaphezulu lebhodlela ngo-75% wotshwala bese uwudlulisela e-super Net Taiwan.
5. Sebenzisa i-pipette (noma i-Pasteur pipette) ukwengeza inani elilinganayo le-serum-containing medium ukuze uqede ukugaya. Pipette kahle ukuze uvumele amaseli ukuthi awe ngokuphelele.
6. Dlulisela uketshezi oluxubile ebhodleleni uye kushubhu ye-centrifuge, ibhalansise, ne-centrifuge cishe imizuzu emi-5.
7. Ngemva kwe-centrifugation, fafaza ingaphezulu leshubhu le-centrifuge ngekani lesifutho lika-alcohol, lidlulisele etafuleni elihlanzeke kakhulu, vula isivalo, bese uthela i-supernatant ethangini likadoti.
8. Engeza ivolumu efanelekile ye-serum-containing medium kanye ne-pipette emnene nge-pipette noma i-Pasteur pipette ukuze wenze ukumiswa kweseli.
9. Hlukanisa ukumiswa kweseli kube amaflaski ahlanzekile ahlanzekile ahlanzekile a-2 (noma ngaphezulu), engeza inani elifanele lendawo yesiko, bese uvala isivalo.
10. Beka flask yesiko elicashuniwe esiteji sesibonakhulu esihlanekezelwe bese ubheka isimo seseli. Inombolo yamaseli kufanele iqinisekiswe. Inombolo encane kakhulu izophazamisa ukukhula.
11. Maka ibhodlela ukukhombisa isikhathi sokudlula, uhlobo lweseli, u-opharetha nolunye ulwazi. Ngaphambi kokulifaka ku-incubator, fafaza ingaphezulu lebhodlela ngotshwala futhi, hlanza itafula lokuhlinza, sula ingaphezulu letafula elihlanzeke kakhulu ngotshwala obungama-75%, futhi uhlanze uketshezi olulahlwayo kanye nodoti.
The ngenhla inqubo ethile yokusebenzisa amaflask amasiko eseli ukudlula amaseli. Qaphela ukuthi kufanele ugqoke izingubo eziyinyumba, amagilavu angenalutho kanye namamaski ngesikhathi usebenza, futhi unake ukusebenza kwe-aseptic kuyo yonke inqubo ukuze ugweme ukungcoliswa kwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.