English
I-cell cryopreservation yindlela ebeka amaseli endaweni enezinga lokushisa eliphansi ukuze kwehliswe i-cell metabolism ukuze igcinwe isikhathi eside. Ngaphezu kokuvikela amaseli, lobu buchwepheshe buvame ukusetshenziselwa ukuthenga, izipho, ukushintshana nokuthutha amaseli athile. Ngakho-ke, siwadlulisela kanjani amaseli a-cryopreserved kokuthi amaflask e-cell culture?
Amaseli aqandisiwe adinga ukuvuselelwa ngaphambi kokuthi kwenziwe umsebenzi olandelayo. Ukuvuselelwa kwamaseli kufanele kusheshe, ukuze ukusebenza kwamaseli kugcinwe kahle. Umsebenzi ungenziwa ngale ndlela elandelayo:
1. Ngemva kokuthola amaseli, hlola ukuthi ukupakisha kwangaphandle kuqedile; vula iphakheji yangaphandle bese ukhipha amaseli agcinwe eqhweni elomile;
2. Hlola ukuthi ishubhu le-cryopreservation lonakele, ukuthi okuqukethwe kuyancibilika yini, bese usheshe ufake ishubhu le-cryopreservation elikhishiwe kubhavu wamanzi ongu-37℃ ukuze ancibilike;
3. Ishubhu ye-cryopreservation ibulawa amagciwane ngotshwala obungama-75% futhi ifakwe kukhabhinethi yokuphepha kwezinto eziphilayo;
4. Engeza u-5ml ophelele wemaphakathi ku-15ml centrifuge tube ozowusebenzisa;
5 Engeza ukumiswa kweseli eshubhuni ye-cryopreservation endaweni elungisiwe bese uxuba kahle;
6. Faka ishubhu le-centrifuge elingu-15mL ku-centrifuge kanye ne-centrifuge ku-1000rpm/min (cishe ama-200g) imizuzu engu-3-5;
7. Engeza i-5ml ephelele ye-medium kuya ku-25ml ye-cellculture flask ukuze uyisebenzise;
8. Khipha amaseli amaphakathi, uwafake inzalo ngotshwala, bese uwafaka ekhabetheni eliphephile;
9. Funa ngomusa i-centrifuged cell supernatant, engeza u-1ml ophakathi nendawo ukuze umise kabusha amaseli;
10. Engeza amaseli amiswe kabusha kufulaski olungisiwe weseli bese uxuba ngobumnene;
11. Bheka isimo kanye nokuminyana kwamaseli ajovwe ngaphansi kwesibonakhulu, bese ubeka iflaski yesiko ku-incubator engu-37°C ukuze uthole isiko. isikhathi, futhi ugweme ukulimala kwamangqamuzana ngenxa yokuncibilika kancane okudala ukuthi amanzi angene kumaseli ukuze enze i-intracellular recrystallization. I-
nezinye izinto ezisetshenziswayo ezisetshenziswayo kufanele zibe amaflask e-aseptic.cell culture flaskscell Culture
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.