English
I-fektri yeselula iyisakhiwo esendlalelo esisodwa noma esendlalelo esiningi sokusebenziseka kwesiko lamaseli. Ngesikhathi senqubo ye-cell culture, sidinga ukubamba isimo sokukhula kwamaseli ngesikhathi ngaphambi kokudlulela esinyathelweni esilandelayo. Inombolo yamaseli asebenzayo ingatholwa nge-trypan blue staining. Indlela ethile yokusebenza imi kanje:
1. Kumaseli abambelelayo, phumula okuphakathi, engeza isixazululo sokugaya, futhi ugaye ku-incubator isikhathi esifanele. Ngemva kokuba amangqamuzana abe yindilinga, ukugayeka kokudla kwaqedwa ngokungeza umthamo olinganayo noma omkhulu we-medium kunomthamo wokugaya. Ngemuva kwe-centrifugation, ukumiswa kweseli kwenziwa nge-culture medium;
2. Thatha isixazululo esiluhlaza se-trypan esingu-0.5ml, i-0.3ml PBS kanye ne-0.2ml cell suspension bese uxuba kahle. Khona-ke ingxube ishiywe i-1-2min. Sebenzisa i-capillary pipette noma ithiphu elincane lepayipi elingu-10ul ukuze udwebe inani elincane lengxube bese ulijova ku-hemocytometer;
3. Bala okungenani amaseli ayi-1000 futhi ubale inani lamaseli anebala;
4. Geza ipuleti lokubala kanye nekhava ngamanzi acwebile kanye ne-ethanol engu-75%;
5. Amaseli avamile ngeke abe namabala, kuphela ngemva kokufa kwamangqamuzana, ukuqina kolwelwesi lwamaseli kuba kukhudlwana, i-trypan eluhlaza okwesibhakabhaka ingena kumaseli, futhi amaseli abonakala eluhlaza okwesibhakabhaka. Ingxube akufanele ibekwe isikhathi eside, ngaphandle kwalokho amangqamuzana aphilayo avamile nawo azothatha udayi, okuzophazamisa ukubala ukunemba;
6. Ukusebenza kweseli (%) = (inani eliphelele lamaseli - inombolo yamaseli anebala)/inani eliphelele lamaseli*100;
7. Njengendlela yokubala yamaseli, i-trypan blue staining inephutha elikhulu. Ingathola kuphela ileveli yokuqala ye-apoptosis, futhi ayikwazi ukubonisa umehluko phakathi kweqembu elilawulayo neqembu lokuhlola.
Enqubweni yokuhlakulela amaseli efekthri yamaseli, kuhlale kukhona amaseli afayo ngenxa yezizathu ezihlukahlukene. Ukwazi inani lamaseli aphilayo kanye nokusebenza kwamaseli kuwumsebenzi obalulekile ekuhlolweni kwesiko lamaseli kanye nendlela ephumelelayo yokuqinisekisa inqubekelaphambili yocwaningo.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.