English
Amaflask empucuko yamaseliziwuhlobo lwezinto ezisebenzisekayo zesiko lamaseli ezivame ukusetshenziswa ekubambeleleni kwesiko lamaseli. Amaseli abambelelayo ahlukile kumaseli amisiwe futhi adinga ukuxhunywa phezulu kwesisekelo ukuze akhule. Umthelela wokubambelela unomthelela ngqo ekukhuleni kwamaseli, futhi umphumela wokubambelela wamaseli ungathuthukiswa ngalezi zindlela ezilandelayo:
1. Ukugaya okufanele kwamaseli: I-HepG-2, A549, Hela, SGC-7901 amaseli omdlavuza angelashwa isikhathi eside. Ngokuvamile, isikhathi sokwelashwa siyimizuzu engu-5-6. I-C2C12, i-COS-7, i-NIH-3T3 kulula ukuwa, futhi imizuzu emi-2 yanele. , i-P19Cl6 kanye namaseli angu-293 awanamatheli ngokuqinile, ngakho-ke angashintshwa afakwe endaweni entsha ngokuqondile ngemva kokuhlanza ku-PBS ukuze awahlakaze.
2. Sebenzisa amabhodlela epulasitiki yamaseli kaningi ngangokunokwenzeka. Ngemva kokuba amabhodlela anjalo aphathwe nge-TC, ukusebenza kokunamathela kwamabhodlela kungathuthukiswa kakhulu, futhi amaseli angaxhunywa kangcono kuwo.
3. Vuselela amaseli amasha futhi usebenzise i-serum engu-20% ukusiza amaseli ukuthi alulame evikini lokuqala.
4. Sebenzisa i-matrix ye-extracellular efanele noma ama-reagents okunamathela ukuze usize amaseli abambelele kangcono odongeni.
5. Zama ukungawanyakazisi amaseli phakathi namahora angu-24 edlule, ukuze ungathinti umphumela wokunamathela kwamaseli.
6. Amaseli e-Epithelial emzimbeni akhula kulwelwesi olungaphansi olwenziwe nge-collagen, ngakho-ke ukutshala ku-substrate nge-collagen kunenzuzo ekukhuleni. Kusikompilo lweseli ye-epidermal cell yomuntu noma yegundane, amaseli angu-3T3 afakwe imisebe ye-γ angasetshenziswa njengongqimba lwe-feeder, futhi i-pH, okuqukethwe kwe-Ca2+ kanye nezinga lokushisa kuyancishiswa, okuzuzisa ukukhula kwamaseli e-epithelial.
Kunezindlela ezimbalwa ukuthuthukisa umthelela wokunamathela wamaseli ku-flask cell culture. Ukwengeza, qiniseka ukuthi usakaza ngokulinganayo ngesikhathi se-subculture ukugwema ukukhula kwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.