English
"Umphumela onqenqemeni" yisimo okuvame ukubhekana naso ngesikhathi sokusetshenziswa kwe-96-well plates, ikakhulukazi kuma-enzyme-linked immunoreactions (ukuhlolwa kwe-ELISA). Lesi simo sizodala ukuthi izinga lokukhula kwamaseli asezimbotsheni ezisemaphethelweni lehle, okuthinta ngqo imiphumela yokuhlolwa.
Lesi simo singasingathwa ngalezi zindlela ezilandelayo:
1. I-Pre-incubation: Sebenzisa ukugeza kwamanzi noma lapho wengeza isisombululo sokusabela epuletini elinomthombo ongu-96, shisisa ipuleti nesixazululo ekushiseni kwe-incubation (okufana no-37°C), okungaqeda kalula "umphumela onqenqemeni" futhi kuthuthukiswe ukuphindaphinda kwesilinganiso. Ukwengeza, olunye ucwaningo luye lwathola ukuthi ukubeka amangqamuzana agandayiwe endaweni yokushisa yasekamelweni ihora elilodwa bese uwabuyisela ku-incubator engu-37°C ukuze aqhubeke nokuhlakulela ngemva kokuba amaseli esezinzile futhi anamathele odongeni nakho kungathuthukisa kakhulu umphumela onqenqemeni.
2. Sebenzisa ikhava evimbela ukuguquguquka: Abanye abantu benze ikhava enezimbobo ezilwa nokuguquguquka ukuze kube nomthelela onqenqemeni obangelwa ukuguquguquka kwe-ipuleti elinezimbotshana. Isembozo sepuleti elinembobo singakha ungqimba lomhwamuko wamanzi ogcwele epuleti elinembobo ukuze lube isivikelo somusi, futhi luhlukanise indawo ezungezile engaphakathi kwepuleti elinembobo endaweni yaselabhorethri, ukuze kugcinwe kangcono ipuleti elinembobo lisesimweni esizinzile ngokuqhathaniswa.
3. Lahla izimbobo ezisemngceleni: Lapho izindlela ezingenhla zingenakuxazululeka, ukuze kutholwe imiphumela yokuhlolwa enokwethenjelwa, indlela eqondile nelula kakhulu ukungasebenzisi lezi zimbobo eziseceleni.
Okungenhla kuyizixazululo zokuvimbela imiphumela enqenqemeni ku-96-well plates. Ukwazi lezi zindlela zokucubungula kunganciphisa umthelela wemiphumela yokuhlolwa.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.