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Amaflask okukhiqiza amaseliziwuhlobo lwezinto ezisetshenziswayo zamaseli ezivame ukusetshenziswa enqubweni yesiko lamaseli. Lapho kukhiqizwa amaseli, ukungcola okuhlukahlukene kuvame ukudala izinkinga ezahlukahlukene zokuhlola futhi kuvimbele inqubo yokuhlolwa. Ngakho-ke singabhekana kanjani nalokhu kungcola?
Ukungcoliswa kwamaseli kuvame ukubangelwa indlela engafanele ye-aseptic, ukuvala inzalo okungaphelele kwezitsha nama-reagents, nokuphasisana kwezandla noma izinsimbi lapho kuvulwe isitsha. Uma amangqamuzana esengcolisiwe, iningi lawo liba nzima ukululama. Okulandelayo yizinhlobo ezijwayelekile nezindlela zokwelapha zokungcoliswa kwamaseli:
1. Ukungcola kwamagciwane: Amagciwane anjengesihlabathi esicolekileyo esimnyama ngaphansi kwesibonakhulu esihlanekezelwe. Ngokuya ngamabhaktheriya anegciwane, angaba nezimo ezihlukene. I-culture medium izoba yi-turbid futhi ibe phuzi, okunomthelela omkhulu ekukhuleni kwamaseli. Iningi lamaseli liyafa phakathi namahora angu-24. Ama-antibiotic ahambisanayo angengezwa endaweni yokukhuliswa, njenge-tetracycline, i-gentamicin, njll. Uma amangqamuzana engewona ayigugu ngokukhethekile, kuyanconywa ukuba uwalahle.
2. Ukungcoliswa kwesikhunta: Ngenxa yokuthi indlela yesiko eflaskini ye-cellculture icacile, ukungcola kwesikhunta kunzima kakhulu ukukubona kusenesikhathi, futhi kuvame ukuba sekwephuzile kakhulu uma kutholwa. Uma amaseli esengcoliswe isikhunta, kuba nzima ukuwagcina. I-Amphotericin B noma iNystatin ngeke isize. Kunconywa ukulahla amaseli angcolile ngokushesha futhi kubulawe amagciwane endaweni ezungezile.
3. Ukungcoliswa kwezikelemu ezimnyama: Izikelemu ezimnyama zingamagciwane ezinga le-nano, amachashazi amnyama ekukhulisweni okuphansi, futhi izimbungulu ezimnyama zingabonakala zibhukuda ngokukhuliswa okuphezulu. Indawo yesiko ayinalo udaka, futhi ngokuvamile ayiyithinti kakhulu. Kodwa uma kuningi kakhulu, kuzophinde kuthinte ukukhula kweseli kanye nemiphumela yokuhlola. Izinga lokusinda kwamaseli lingathuthukiswa ngokwandisa ukuminyana kwamaseli.
4. Ukungcola kwe-Mycoplasma: I-Mycoplasma ayibonakali ngaphansi kwesibonakhulu esihlanekezelwe. Esigabeni sokuqala sokungcoliswa, isiko lamasiko alibi. Ngemuva kokungcola kwe-mycoplasma, kungathinta yonke imingcele yokukhula kwamaseli. Uma ukungcola okunjalo kutholwa, kutuswa ukuthi kulahlwe amaseli bese uphinde ukuhlola.
Okungenhla kunezindlela ezimbalwa ezijwayelekile zokungcola nezindlela zokwelapha amaflask amasiko weseli. Ukuvimbela ukungcoliswa kwamangqamuzana kufanele kube yikona okugxilwe kakhulu kukho, futhi igumbi lamasiko, izitsha noketshezi lwamasiko kufanele kubulawe amagciwane futhi kubulawe amagciwane. Lapho usebenza, sebenzisa ngokuqinile ukucaciswa kokusebenza kwe-aseptic.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.