English
Isiko lamaseli libandakanya ukujova kwamaseli, ukutshala, ukuvuna kanye nezinye izinqubo. Amaseli ku-ama-cell culture flasks akhule aze afike ezingeni elithile futhi amaseli adinga ukuvunwa. Ngalesi sikhathi, amaseli asodongeni lweflask adinga ukugaywa nge-trypsin bese evunwa. Isikhathi sokugaya kufanele silawulwe ngesikhathi kusetshenziswa i-pancreatin.
I-Trypsin iyi-protease ehlukahlukene, engehlisa isithunzi samaprotheni kulwelwesi lwamaseli kanye namaflaski e-cell culture. Ngokululaza amaprotheni endaweni ethile, iphrotheni ekuhlanganeni phakathi kwamaprotheni ayonakaliswa, futhi amangqamuzana aba yindilinga ngenxa yokuqina kwe-cytoskeleton yawo yangaphakathi, ngaleyo ndlela ahlukanise amaseli. Izinga lokugaya i-trypsin liyisinyathelo esibalulekile esikweni lamangqamuzana: imfucumfucu yamangqamuzana egayiwe ngokweqile iyanda, udoti omnyama uyakhula, amangqamuzana azowa abe yizicucu, okuthinta kakhulu ukusebenza kwamangqamuzana, futhi amanye amangqamuzana antante futhi alahlekelwe yi-trypsin elahliwe; ukugaya akwanele, amaseli anzima ukuwaqhumisa esuka odongeni lwebhodlela, ukubhoboza ngamapayipi okuphindaphindiwe kuzolimaza nokusebenza kwamaseli.
Izicubu noma amaseli ahlukene asabela ngendlela ehlukile esenzweni se-pancreatin. Umsebenzi wamaseli ahlakazekile we-pancreatin nawo uhlobene nokugxila kwawo, izinga lokushisa kanye nesikhathi sokusebenza. Lapho i-pH ingu-8.0 nezinga lokushisa lingu-37, amandla okwenza isixazululo se-pancreatin anamandla uma kuqhathaniswa. Lapho usebenzisa i-pancreatin, ukugxila, izinga lokushisa kanye nesikhathi kufanele kulawulwe ukuze kugwenywe ukulimala kwamaseli okubangelwa ukugaya ngokweqile. Ngenxa yokuthi i-Ca2+, Mg2+, serum kanye namaprotheni kunganciphisa umsebenzi we-pancreatin, i-BSS ngaphandle kwe-Ca2+ ne-Mg2+ kufanele isetshenziswe lapho kulungiswa isisombululo se-pancreatin, njengesixazululo se-D-Hanks. Lapho unqamula ukugaya, sebenzisa i-serum-containing culture medium noma i-trypsin inhibitor ukumisa umphumela we-pancreatin kumaseli.
Ngaphezu kwalokho, uma kuyingqamuzana elisanda kuthengwa, ungasebenzisa kuqala ukugcwala okuphansi kwe-trypsin ukuze uthole ukugaya. isikhathi, futhi ubheke ukuthi amaseli ayindilinga njalo ngomzuzu futhi enze irekhodi. Ngamafuphi, lapho uvuna amaseli ku-amaflask amasiko amaseli, isikhathi sokugaya i-trypsin kufanele silawulwe ukuze kugcinwe ukusebenza kwamangqamuzana.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.