English
Enkambweni yokukhiqiza amaseli, ukushintsha okuphakathi kuwumsebenzi ojwayelekile, osetshenziswa kakhulu ukususa imfucuza ehlukahlukene ye-metabolic eyenziwa ngesikhathi sokukhula kwamangqamuzana, ukwengezela isiko elisha, nokukhuthaza ukukhula kwamangqamuzana. Ngakho-ke, ungasishintsha kanjani isixazululo ku-amaflask e-cell culture .?
Izinyathelo ezithile:
1. Faka okokusebenza etafuleni elihlanzeke kakhulu futhi uvule imisebe ye-ultraviolet ukuze uvale amagciwane. Uma amaseli akhulisiwe ezwela izinga lokushisa, ungafaka ibhodlela eliphakathi nendawo eliqukethe i-serum kubhavu wamanzi ongu-37℃ ukuze wenze izinga lokushisa eliphakathi nendawo libe ngu-37℃, bese udlulisela Iya ebhentshini elihlanzeke kakhulu lokuvalwa kwe-UV. Ngaphezu kwalokho, igumbi le-cell Culture lidinga nemisebe ye-UV cishe isigamu sehora.
2. Khipha ama-flask of cell culture ku-incubator, fafaza u-75% utshwala phezu kwebhodlela ngekani le-alcohol spray ukuze kubulawe amagciwane, udlulisele etafuleni elihlanzekile kakhulu, uvule isivalo, ukhiphe ngobumnene isiko elidala, sebenzisa i-pipette ukuze wengeze i-serum entsha equkethe i-serum, Qaphela ukuthi ungangezi i-culture medium kusuka endaweni enamathelayo yamaseli, kodwa kusukela odongeni oluseceleni, ngobumnene, ukuze ungawashi amaseli.
3. Ngemva kokwengeza i-culture medium, vala isivalo bese umaka ibhodlela ukukhombisa isikhathi sokushintsha okuphakathi, uhlobo lweseli, opharetha nolunye ulwazi.
4. Ngaphambi kokulifaka ku-incubator, fafaza ingaphezulu lebhodlela ngotshwala futhi, bese ukhumbula ukuqoqa itafula lokuhlinza, usule ingaphezulu letafula elihlanzeke kakhulu ngotshwala, futhi uhlanze uketshezi olungcolile kanye nodoti.
Lapho ushintsha uketshezi, naka inzalo kuyo yonke inqubo. Ama-opharetha kufanele agqoke izingubo eziyinyumba, amagilavu angenalutho namamaski, futhi baqaphele lapho bethinta umlomo we-
ngezandla zabo ukuze bagweme ukungcola.cell culture flasksamaflask e-cell culture
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.