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2. Izinto kanye nezindlela
2.1. Ukugcinwa kwe-Huh7.5 Cells
Huh7.5 cell cultures inakekelwe kokuthi cell culture T flasks maphakathi ne-serum-containing (SCM): I-Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, UK) equkethe 4 mM GlutaMAX futhi yengezwe ngokuthi 10% i-fetus bovine serum (Sigma, St. Louis, MO, USA), 100 U/mL penicillin kanye ne-100 µg/mL streptomycin (Sigma). Kumaflask e-T175 (i-Nunc, i-Roskilde, i-Denmark) kanye nama-flasks amasiko we-triple layer (Nunc), amaseli we-Huh7.5 ayedluliswa njalo ezinsukwini ezingu-2-3. Wonke amasiko amaseli agcinwe ku-37 °C naku-5% CO2.
2.2. I-HCV Virus Stocks
Izitoko ezimbili eziqinisekisiwe ngokulandelana kwe-genotype 5a recombinant SA13/JFH1core-NS5B [28], ezitshalwe ku-monolayer Huh7.5 cell cultures kumaflask e-T, zisetshenziswe kulolu cwaningo. Isitokwe segciwane lesiqephu sesithathu esine-infectivity titer engu-5.8 log10 FFU/mL, ekhule ngaphansi kwezimo eziqukethe i-serum (SC) [39], sasetshenziselwa ukujova amasiko e-CelCradle™, ukunyakazisa amasiko eflask, kanye nokuhlolwa kokubonakala kwegciwane. Isitokwe segciwane lesiqephu sesine esine-infectivity titer engu-5.1 log10 FFU/mL [32], etshalwe ngaphansi kwezimo ze-SF [39], sasetshenziselwa ukuhlola ukulinganiswa kwegciwane.
Umthombo kusuka: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.