English
Ukungcoliswa kwe-Mycoplasma kuyinkinga evame kakhulu lapho uhlakulela amaseli kokuthi izimboni zamaseli. Ngokungafani nokunye ukungcola, amangqamuzana angcoliswe yi-mycoplasma ngokuvamile awabi namafu, ngakho kunzima ukwahlulela ngeso lenyama. Kunezindlela ezine zokunquma ukuthi amaseli angcolile yini nge-mycoplasma:
1. I-DNA fluorescence staining
Indlela ye-DNA fluorescence staining isekelwe kumgomo wokuthi udayi we-fluorescent i-Hoechst33258 ingabophezela endaweni ecebile ngesisekelo se-AT ku-DNA ye-mycoplasma. Amachashazi e-Fluorescent ayi-mycoplasma DNA ecebile ezifundeni eziyisisekelo ze-AT.
2. PCR technology
Iziqalo ezithile ziklanywe ngokulandelana okulondoloziwe ku-Mycoplasma genome, i-nucleic acid yesampula ezohlolwa iyakhuliswa, futhi ukuxilongwa kwenziwa ngokuhlaziya usayizi womkhiqizo okhulisiwe. Ubuchwepheshe bokuthola i-PCR busetshenziselwa ukutholwa kokungcola kwe-mycoplasma, ngomjikelezo omfushane, ukuzwela okuphezulu, ukucaciswa okuhle, ukusebenza okulula, futhi kungathola inani elikhulu lamasampuli ngesikhathi esisodwa.
3. I-Enzyme-linked immunosorbent assay (ELISA)
ELISA isetshenziswa ekutholeni ukungcola kwe-mycoplasma, ngokucaciswa okuhle nokuzwela, futhi ingaqedela ukutholwa kwenani elikhulu lamasampuli ngesikhathi esisodwa. Inezici zokutholwa kwekhwalithi okulula, okusheshayo kanye nomthamo.
4. Isibonakhulu se-electron
Ngokujwayelekile, amaseli ezimbonini zamaseli akhuliswa amahora angama-48 kuye kwangama-72. Ngaphambi kokuthi amaseli acishe ahlangane, amaseli agaywa nge-trypsin ukuze enze ukumiswa kweseli, kulungiswe, kushumekwe, futhi kusikwe ngaphambi kokubhekwa.
Okungenhla izindlela ezine ezivame ukusetshenziswa lapho kutholwa ukungcoliswa kwe-mycoplasma ezimbonini zamaseli. Njengamanje, kunezinhlobo ezingaphezu kuka-20 ze-mycoplasma ezaziwa ngokungcolisa amangqamuzana, okungaphezu kuka-95% okuyi-mycoplasma yomlomo, ngakho-ke opharetha kufanele banake ukusebenza kwe-aseptic lapho behlakulela amaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.