English
I-cell factory iyisakhiwo sezendlalelo eziningi zezinto ezisetshenziswayo zesiko lamaseli. Ukucaciswa okujwayelekile kufaka ungqimba olu-1, izendlalelo ezi-2, izendlalelo ezi-5, izendlalelo eziyi-10, izendlalelo ezingama-40, njll. Uma inani lezendlalelo liphezulu, indawo yesiko iba nkulu. Lokhu okusebenzisekayo kusetshenziswa kakhulu isiko lamaseli abambelelayo, lapho izimo ezilandelayo zidingeka ekukhuleni kweseli:
1. Sterile environment
Ubuthi kanye nenzalo yizimo ezibalulekile zokulima amaseli ezimbonini zamaseli. Ku-vivo, isistimu ye-detoxification kanye namasosha omzimba angamelana nokuhlasela kwamagciwane noma ezinye izinto eziyingozi, kodwa inqubo ye-in vitro isiko, amangqamuzana aswele ukuvikelwa kwamasosha omzimba futhi alahlekelwe amandla okuzivikela kuma-microorganisms futhi akhiphe izinto eziyingozi. . Ukuqinisekisa ukuthi amaseli angakhula futhi akhiqize kabusha endaweni ye-in vitro, kuyadingeka ukuthi kuqinisekiswe indawo yokusebenza eyinyumba, inhlanzeko yomuntu siqu, izinto ezivuselela inyumbazane nemidiya, nokuphathwa kwe-aseptic.
2. Izinga lokushisa elilungile
Ngokujwayelekile, izinga lokushisa elifanele lamaseli ezilwane ezincelisayo kanye nezinyoni zezinyoni ukuze akhuliswe ku-vitro ngu-37-38°C. Izinga lokushisa le-ambient elingafanele lizothinta ukukhula kwamaseli. Ukubekezelelana kwamaseli ekushiseni okuphansi kunamandla kunokushisa okuphezulu. Emazingeni okushisa aphansi, umsebenzi we-metabolic kanye namandla e-mitotic amaseli kuyehla. Uma izinga lokushisa lingekho ngaphansi kuka-0 °C, nakuba i-cell metabolism ithinteka, akukho monakalo; ku-25 kuya ku-35 ° C, amangqamuzana akhula ngesilinganiso esincane; kodwa uma ibekwe ku-40 °C amahora ambalwa, akugcini nje ngokungathandeki ekusindeni kweseli nokukhula. , futhi kuholele nasekufeni kwayo.
3. Ingcindezi ye-osmotic efanelekile
Izixazululo ze-Hypertonic noma ze-hypotonic zingabangela amaseli ukuthi agoqeke, avuvuke, futhi aqhume. Ngakho-ke, ingcindezi ye-osmotic ingenye yezimo ezibalulekile zokutshala amaseli ku-vitro. Amaseli amaningi akhuliswe ku-vitro anokubekezela okuthile kungcindezi ye-osmotic. Ezisetshenziswa ezingokoqobo, umfutho we-osmotic ongu-260-320 mmol/L ungasetshenziswa kumaseli amaningi.
4. Indawo yegesi kanye ne-pH
Isiko le-in vitro lamaseli lidinga indawo ekahle yegesi. Umoya-mpilo kanye ne-carbon dioxide yizimo ezidingekayo ukuze amangqamuzana aphile. I-oksijeni ibamba iqhaza kumjikelezo we-tricarboxylic acid yamaseli, ihlinzeka ngamandla okusinda kwamangqamuzana, i-metabolism kanye nokuhlanganiswa; i-carbon dioxide ayiyona nje i-metabolite yamaseli, isakhi esibalulekile ekukhuleni kwamaseli, kodwa futhi ihlobene nokugcina i-pH yendawo yesiko. Ibanga le-pH elifanele lamaseli amaningi livamise ukuba ngu-7.2 kuya ku-7.4. Esikweni elivulekile, isilinganiso segesi se-carbon dioxide esingu-5% sifanelekile.
Okungenhla yizimo ezine ezinkulu zokutshala amaseli ezimbonini zamaseli. Lezi zimo ezemvelo. Ukwengeza, ukukhula kwamangqamuzana nakho kudinga ukwengeza i-serum, i-culture medium kanye nezinye izakhamzimba ezidingekayo ekukhuleni kwamaseli, okuyisici esibalulekile sokuqinisekisa ukukhula kwamaseli okuvamile.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.