I- ama-cell culture flasks isetshenziswa kakhulu enqubweni yesiko lamaseli, elungele kakhulu isiko lamaseli esikali esimaphakathi kanye nezicubu elabhorethri. Ukusebenzisa i-polystyrene eluhlaza, ukucaciswa okujwayelekile kufaka phakathi u-25cm2, 75cm2, 175cm2 kanye no-225cm2, njll. Ngokusho kwenqubo yokwelashwa kwendawo, ihlukaniswe yaba i-hydrophilic (efanele isiko lamaseli anamathelayo) kanye ne-hydrophobic (efanele isiko leseli lokumiswa).
Ukukhula kweseli kudinga indawo ethile, njengokungazali, ingcindezi ye-osmotic, izinga lokushisa, i-pH, njll. Ukuze ivumelane nokukhula kwamangqamuzana, amaflask ayisikwele e-cell culture anezici ezilandelayo:
1. Ukusuka ekubunjweni komjovo ukuya ekubunjweni komhlangano, amabhodlela e-cell culture akhiqizwa endaweni yokusebenzela yokuhlanza izinga le-C. Ukukhiqizwa kuqedwa ngemishini ezenzakalelayo ngokuhambisana namazinga e-cGMP. Akukho ukuxhumana okuqondile nabasebenzi, futhi umkhiqizo unokuvumelana okuhle, awekho ama-pyrogens, futhi awekho izithako ezisuselwe ezilwaneni.
2. Inqubo yokuguqulwa kwe-vacuum plasma ithuthukisa ukusebenza kokunamathela kwamaseli, futhi ifaneleka ekukhuleni kwamangqamuzana anezici zokunamathela eziphansi.
3. Idizayini yesitaki akulula ukuyishelela, kulula ukuyipakisha, futhi kulula ukuyiphatha.
4. Indawo engaphansi yebhodlela iklanywe ngendlela eyindilinga eyi-arc, enciphisa i-engeli efile yokuthintana lapho utshani buphephetha kumaseli.
5. Umkhiqizo ojwayelekile ufakwe i-0.2μm hydrophobic filter membrane coverable breathable kanye nesembozo sokuvala, esingasetshenziswa ezindaweni ezihlukahlukene zamasiko. Ikhava ephefumulayo isetshenziswa endaweni ye-CO2, futhi isembozo sokuvala singasetshenziswa kuma-incubator ajwayelekile nasezindlini zokugcina izithombo ezingenayo i-carbon dioxide.
6. Isivalo sebhodlela sisebenzisa ulwelwesi oluphefumulayo lwe-hydrophobic, olungeke luthinte umgoqo we-microbial kanye nomthelela wokuphefumula we-membrane ephefumulayo ngemva kokuthintana noketshezi.
Lokhu okungenhla yizici zeflask ye-cell culture. Noma ngabe imvelo yokukhiqiza, ukwakheka kwekepisi noma okuphansi, noma amakepisi ahlukene, zonke kufanele zivumelane nezimo nezidingo zamaseli ahlukene, futhi ziqhubekisele phambili inqubekelaphambili yomsebenzi wosikompilo lwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.