English
Ukungcoliswa kwamaseli kuyinkinga enzima kakhulu lapho uhlakulela amaseli. Uma amaseli esengcolisiwe, amaseli azokhula kancane, futhi ezimeni ezinzima, azoholela ekufeni kweseli futhi athinte inqubo yokuhlola. Ukungcoliswa komzimba kuwuhlobo oluvamile lokunukubezeka lapho uhlakulela amangqamuzana kokuthi amafekthri amaseli.
Ukungcola komzimba ikakhulukazi kubhekisela ekubhujisweni kwamangqamuzana ngenxa yezinto ezibonakalayo ezifana nezinga lokushisa, ukudlidliza, imisebe, nemisebe. Izinga lokushisa elilungile lamaseli amaningi azokhuliswa ku-invitro ngu-37-38°C. Emazingeni okushisa aphansi, umsebenzi we-metabolic namandla we-mitotic wamaseli uyancishiswa. Uma izinga lokushisa lingekho ngaphansi kuka-0 °C, nakuba i-cell metabolism ithinteka, akukho monakalo; ku-25 kuya ku-35 ° C, amangqamuzana akhula ngesilinganiso esincane; kodwa uma ibekwe ku-40 °C amahora ambalwa, akugcini nje ngokungathandeki ekusindeni kweseli nokukhula. , futhi kungaholela ngisho nasekufeni kwamangqamuzana ngaphakathi kwemboni yamaseli. Ngesikhathi esifanayo, i-medium, i-buffer, i-fetal bovine serum, njll. esetshenziselwa ukukhiqizwa kwamaseli kufanele ijwayele ukusetshenziswa kumaseli lapho izinga lokushisa liku-37°C, ukuze kugwenywe ukulimala kwamaseli ngenxa yokushisa okuphansi.
Ngaphezu kwalokho, uma i-cellculture medium, njll. ivezwe emisebeni yemisebe noma ye-ultraviolet, izodala izinguquko kumetabolism yamangqamuzana. Kunemishini ekwazi ukukhiqiza ukudlidliza komshini eduze kwe-incubator yokushisa engashintshi, okuzokwenza ukuthi i-cytoplasm igeleze ngokungahambi kahle, futhi ukwakheka kwe-colloidal yama-protoplast nakho kuzoshintsha kakhulu. Uma ukudlidliza kudlula inani elithile, kuzoholela ekulimaleni kweseli nokungalungi kwekhromozomi. Ngakho-ke, kutuswa ukuba ungabeki okokusebenza okudlikizayo eduze kwama-incubator e-carbon dioxide.
Lokhu okungenhla ukungcola okujwayelekile komzimba lapho kutshalwa amangqamuzana ezimbonini zamangqamuzana. Amaseli ayazwela endaweni ezungezile, futhi noma iluphi uhlobo lokungcola lungase luthinte ukukhula nokukhiqizwa kwawo. Ngakho-ke, ukusebenza kufanele kwenziwe ngokuhambisana ngokuqinile nezinqubo ezinqunyiwe zokugwema ukwethula imithombo yokungcola.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.