English
I-cell factory iwuhlaka olunezendlalelo eziningi zezinto ezisebenzisekayo zesiko lamaseli, ikakhulukazi ezisetshenziselwa isiko elikhulu le-in vitro cell. Lapho kulima amangqamuzana, indawo ethile iyadingeka, futhi imvelo yegesi kanye nenani le-pH kungenye yezimo ezibaluleke kakhulu.
Indawo yegesi ifektri yamaseli ebhekisela kuyo lapho kutshalwa amangqamuzana ikakhulukazi i-carbon dioxide futhi umoya-mpilo. Umoya-mpilo ubamba iqhaza kumjikelezo we-tricarboxylic acid weseli ukuze ukhiqize amandla okukhula kwamangqamuzana, ukwanda kanye nokuhlanganiswa kwezingxenye ezihlukahlukene ezidingekayo. Amanye amaseli angathola amandla nge-glycolysis ngaphansi kwezimo ze-hypoxic, kodwa amaseli amaningi awakwazi ukuphila ngaphansi kwe-hypoxia. Ukungezwani komoyampilo kuvamise ukugcinwa ngaphansi kwezimo zomkhathi, futhi uma ukucindezela kwengxenye komoyampilo kudlula inani lomoya-mpilo osemkhathini, amanye amaseli angase abe yingozi. Uma usebenzisa isiko elivulekile (isiko lesitsha noma isiko lebhodlela lesiko noma isiko lepuleti lesiko), amaseli ngokuvamile abekwa endaweni exubile yegesi engu-95% yomoya kanye no-5% we-carbon dioxide.
I-Carbon dioxide ayiyona nje i-metabolite ye-metabolite amaseli, kodwa futhi ingxenye ebalulekile ekukhuleni kwamaseli, futhi ihlobene nokugcina i-pH yendawo yesiko. Endabeni yokuhlushwa kwe-carbon dioxide ephansi endaweni evaliwe, amaseli kulula ukukhula; ngokuvamile ayikwazi ukuba ngaphansi kuka-1%, ngaphandle kwalokho amaseli azolimala. Uma isiko elivulekile libekwe endaweni yegesi equkethe u-5% we-carbon dioxide, kuyancomeka.
Amaseli amaningi afanele ukukhula ku-pH 7.2~7.4, aphansi kune-pH 6.8 noma ngaphezulu kune-pH 7.6 ayingozi kumaseli, futhi ngisho uwohloka noma ufe. Amaseli ahlukene anezidingo ezahlukene zevelu ye-pH. Ngokuvamile, amangqamuzana amasiko ayisisekelo awabekezeleli kancane inani le-pH ye-alkali kune-asidi, futhi indawo ene-asidi inenzuzo kakhulu ekukhuleni kwamangqamuzana kunendawo ene-alkaline. Ukuze kugcinwe inani le-pH eliqhubekayo lendawo yesiko, indlela yokwengeza isigcinalwazi esifana ne-phosphate endaweni ivame ukusetshenziswa. amasimu. Kodwa kungakhathaliseki ukuthi iyiphi insimu esetshenziswa kuyo, imvelo yegesi kanye nenani le-pH phakathi nesiko lamaseli kusengenye yezimo ezibalulekile zokukhula kwamaseli.
With the rapid development of the field of life science, cell factories are being applied to more fields. But no matter which field it is used in, the gas environment and pH value during cell culture are still one of the important conditions for cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.