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Ukusebenza kahle okuphezulu erlenmeyer shake flask kuwumkhuba wamaseli ojwayelekile ukusetshenziswa, ikakhulukazi esetshenziselwa ukutshala amaseli ezilwane ezincelisayo, amaseli ezinambuzane namanye amaseli amisiwe. Phakathi kwazo, i-CHO cell antibody pharmaceutical iyingxenye ebalulekile yenkundla yokusetshenziswa kwayo.
CHO amaseli aqala ukuhlukaniswa ilabhorethri ye-Puck ngo-1957 futhi atholwa ngokugayeka kwe-enzymatic kuka-0.1 g we-Chinese hamster ovary tissue. Ngenxa yezinguquko eziyinkimbinkimbi zangemuva kokuhumusha ezifana nezabantu, kanye nokukhula kokumiswa okusheshayo nokuqinile kwemidiya engenayo i-serum nechazwa ngamakhemikhali, amaseli e-CHO avame ukusetshenziselwa ukuveza amaprotheni e-biological macromolecular asebenzayo. Njengamanje, cishe i-70% yamaprotheni okwelapha athengisiwe aboniswa futhi akhiqizwa amaseli e-CHO, futhi amaseli e-CHO abe yithuluzi eliyinhloko lokukhiqiza amaprotheni okwelapha adinga ukuguqulwa okuyinkimbinkimbi ngemva kokuhumusha. Isibonelo, umuthi wokugomela i-coronavirus omusha (i-CHO cell) oqalwe kabusha ngoMashi 2021 wasungulwa usuka kumaseli e-CHO. Umgomo uwukuhlanganisa kabusha isakhi sofuzo esisha se-coronavirus S protein receptor binding region (RBD) sibe amangqamuzana e-Chinese hamster ovary (CHO) cell, i-invitro iveze i-RBD dimer, futhi kwengezwe i-aluminiyamu hydroxide adjuvant ukuze kuthuthukiswe amandla omzimba .
Ekuqaleni isigaba socwaningo lwezemithi lwe-CHO cell antibody, amaflaski e-erlenmeyer shake asebenza kahle kakhulu ayizinto ezisetshenziswayo ezisetshenziswayo. Ngemuva kokuthi amangqamuzana avuselelwe akhuliswe ku-flask ye-shake esebenza kahle kakhulu izinsuku ezingu-2-3, ukuminyana kwamaseli kuyanda futhi imisoco iyadliwa kancane kancane. Ngalesi sikhathi, amaseli adinga ukunwetshwa. Bala ivolumu yokunweba ngokuya ngokuminyana kwamaseli. Lapho inani lamaseli azojovwa ku-reactor lifinyelelwa, misa isiko le-shake flask, gcoba i-reactor ukuze uthole isiko, futhi uvune amangqamuzana ngemva kokuqedwa kwesiko.
Ikhemisi le-CHO cell antibody liyingxenye eyodwa kuphela yohlelo ukusebenza kahle okuphezulu erlenmeyer shake flasks. Ukwengeza, lokhu okusebenzisekayo kudlala indima ebalulekile ocwaningweni lwamasosha omzimba we-monoclonal kanye nokuvutshelwa nokutshalwa kwebhaktheriya ezimbonini zobisi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.