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The cell Culture plateiwuhlobo lwethuluzi lebhayoloji. Ihlukaniswe yaba phansi okuyisicaba kanye nephansi eliyindingilizi ngokuya ngokuma. Inombolo yemithombo ihlanganisa imithombo engu-6, imithombo engu-12, imithombo engu-24, 48 imithombo, imithombo engu-96, imithombo engu-384, njll. Isetshenziselwa ikakhulukazi isiko lamaseli futhi ingasetshenziswa futhi ukukala. ukugxila kwamaprotheni, amapuleti angu-96-well avame ukusetshenziselwa ukutholwa kwe-MTT.
indlela ye-MTT, eyaziwa nangokuthi indlela ye-MTT colorimetric, iyindlela yokuthola ukusinda nokukhula kwamaseli. Umgomo wokuthola ukuthi i-succinate dehydrogenase ku-mitochondria yamaseli aphilayo inganciphisa i-MTT yangaphandle ibe yicrystal formazan (i-Formazan) engancibiliki eluhlaza okwesibhakabhaka futhi iyifake kumaseli, kuyilapho amaseli afile engenawo umsebenzi onjalo. I-Dimethyl sulfoxide (DMSO) ingancibilikisa i-formazan kumaseli, futhi inani layo lokumunca ukukhanya likalwa ku-570nm nge-enzyme-linked immunoassay, engabonisa ngokungaqondile inani lamaseli aphilayo. Phakathi kohlu oluthile lwezinombolo zamaseli, inani lokwakheka kwekristalu ye-MTT lilingana nenani lamaseli. Izinyathelo zokuthola zimi kanje:
1.
Yenza amaseli kokuthi 96-well cell culture plate. Ngemuva kokuthi ukuminyana kwamaseli kufaneleka, engeza u-50ul MTT emthonjeni ngamunye futhi uqhubeke nokucula amahora angu-4;
2.
Ncela indlela yesiko eliqukethe i-MTT bese wengeza u-150ul we-DMSO. Dlidliza uvundlile imizuzu eyi-10 ukuze uncibilike ngokuphelele amakristalu. Kumaseli amisiwe, ngemva kwe-centrifugation, suck away the medium, bese wengeza i-DMSO ukuze incibilike;
3. Linganisa ukumunca kokungu-550nm ethuluzini le-immunoassay elixhunywe ku-enzyme (setha iqembu elinengqondo lokulawula neqembu elingenalutho);
4. I-cell survival rate=inani lokumunca leqembu lokuhlola/inani lokumunca leqembu lokulawula*100%.
Kufanele kuqashelwe ngesikhathi sokusebenza ukuthi i-MTT ibangela umdlavuza. Uma kungenzeka, zama ukugqoka uhlobo lwamagilavu efilimu asobala. I-MTT elungisiwe idinga ukuba inyumba. I-MTT izwela kakhulu kumagciwane. Akunandaba ukuthi wengeza kupuleti le-96-well cell culture ngaphandle kokulivikela ekukhanyeni. , Ngemuva kwakho konke, isikhathi sifushane, noma ungacisha ukukhanya ku-console.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.