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Amaflask e-Cell Cultureziwuhlobo lwezinto ezidliwayo zesiko lamaseli ezisetshenziswa kakhulu emakethe, futhi zifaneleka kakhulu isiko lamaseli abambelelayo. Phakathi kwazo, lokhu okusebenzisekayo kuyasetshenziswa nasekudluleni kwe-mouse fibroblasts.
Ama-Mouse fibroblasts amaseli akhethekile asetshenziselwa izinjongo okungezona ezezokwelapha njengocwaningo lwesayensi noma izinhlelo zokusebenza zezimboni. Ama-Fibroblasts anemisebenzi enamandla kanye ne-basophilic cytoplasm ebuthakathaka. Banomsebenzi ocacile wokuhlanganisa amaprotheni kanye nemisebenzi yokufihla. Ngaphansi kwezimo ezithile, Angakwazi ukufeza ukuguqulwa okufanayo namaseli e-fiber. Ngemuva kokuthola ama-fibroblasts, adinga ukuqiniswa ku-incubator cishe amahora angu-4 bese ekhuliswa noma adluliselwe ngokusho komthamo wamaseli, ukushintsha okuphakathi. Umsebenzi wokudlulisa ulandela lezi zinyathelo ezilandelayo:
1. Hlaziya okuphakathi kufulaski wangempela weseli, hlambulula amaseli kabili nge-PBS buffer, bese wengeza u-1~2 ml ka-0.25% EDTA wokugaya kwe-trypsin (naka isikhathi sokugayeka kokudla, ngokuvamile esilawulwa phakathi kuka-1~2min).
2. Bheka ukugaya ngaphansi kwesibonakhulu. Lapho unqenqema lweseli luncipha futhi lunamathela odongeni (ukugaya akunconywa kuze kube yilapho amangqamuzana entanta), khipha i-trypsin, engeza u-6~8ml we-medium ephelele, uvunguze ngobumnene ungqimba lweseli, bese uzama ukuphephetha bese uphephetha ungqimba lweseli. .
3. Khipha ingxenye yokulengiswa kweseli bese uyidlulisela kuflask entsha ye-cell culture, engeza i medium ephelele efanele, bese uhlanyela ku-incubator.
4. Naka ukuguqulwa kwevelu ye-pH yendawo ephakathi, shintsha okuphakathi njalo, bese uphinda i-subculture noma isitoreji esiqandisiwe lapho ukuminyana kwamaseli kufinyelela ku-70-80%.
Indima enkulu ka-amaflask e-cell culture ekudlulisweni kweseli gcina amaseli futhi unikeze indawo yokukhula enamathelayo yamaseli. Kufanele kuqashelwe ukuthi uma udonga lwamaseli luphukile noma luvuza ngemva kokuthola amaseli, kusho ukuthi amaseli angcolile, futhi umphakeli kufanele athintwe kusenesikhathi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.