English
Amabhaktheriya e-Lysogenic abhekisela kubhaktheriya anama-prophage ahlanganiswe kuma-chromosome awo futhi angakhula futhi akhiqize ngokujwayelekile ngaphandle kokuthi i-lysed. Ama-bacteriophage athambile asetshenziswe kabanzi emikhakheni yokudluliselwa, ama-vector wobunjiniyela bezakhi zofuzo, kanye nebhayoloji yamangqamuzana. I-i-cell culture flask ingasetshenziswa ukuhlola i-lysogenicity yamagciwane. Indlela ethile yokusebenza imi kanje:
1. Isiko lamabhaktheriya e-lysogenic: Inoculate E. coli 225 (λ) eyenziwe i-LB slope ibe yifulaski ye-cell culture engu-250ml equkethe isisombululo se-20ml LB, isiko ngokuthuthumela ku-30 ° C amahora angu-16, bese ithatha u-2ml ukumiswa kwebhaktheriya kuyo futhi ixhume kwelinye Eflaskini ye-Erlenmeyer engu-250ml equkethe u-20ml womhluzi we-LB, isiko elinokunyakaziswa ku-37°C amahora angu-2 ukuya esigabeni se-logarithmic.
2. Ungafaki i-free phage: Uma uhlobo oluzohlolwa luyi-Bacillus, lungenziwa ukumiswa kwe-spore, bese luphathwa ku-80 ° C imizuzu engu-10 ukuze kubulawe i-phage engase ikhangiswe futhi ikhululeke ebusweni bebhaktheriya ye-lysogenic; uma uhlobo oluzohlolwa lungeyona i-Bacillus , Sebenzisa i-antiserum elungiselelwe i-phage noma i-0.2% yesisombululo se-sodium citrate ukuze ugeze amaseli we-log yesigaba se-lysogen ukuze ususe i-surface-adsorbed kanye ne-phage yamahhala. Ngemuva kwe-centrifugation ku-4000r/min imizuzu engu-10, i-supernatant ingangezwa ngamabhaktheriya ezinkomba ezibucayi ukuze kunqunywe ipuleti eliphindwe kabili, elisetshenziselwa ukuhlola i-phage yamahhala yesampula kanye ne-phage ekhangisa amagciwane e-lysogenic. I-phage ngemva kwe-centrifugation Amaseli ayayengwa.
3. Ukungeniswa kwamabhaktheriya e-lysogenic: amagciwane aqoqwe ngemva kokufakwa phakathi ahlanjululwa kabili ngosawoti ojwayelekile oyinyumba, futhi enziwa abe ukuhlangana kokugcina kwamaseli angu-1011/ml anosawoti ovamile noma 100mmol/l Tris-HCL buffer (pH7.0) Ukuze kumiswe amagciwane, engeza. I-5ml yokumiswa kwebhaktheriya esitsheni ngasinye, khanyisa ngesibani esingu-30W se-UV ebangeni elingu-30cm izikhathi ezingu-30, ngokushesha wengeze u-5ml wesisombululo se-double concentration LB, uhlanganise kahle futhi ufukamele ebumnyameni obungu-37°C amahora angu-2.
4. Ukuhlolwa kwezinhlobo ze-lysogenic: Sebenzisa ngokwendlela ye-agar enezingqimba ezimbili, engeza amaconsi ambalwa e-chloroform ku-lysate eyenziwe ngenhla, futhi uhlambulule ngokufanelekile ngendlela yokuhlanza eyi-10. Ngemuva kokukhetha ukuhlanjululwa okuthathu kwe-dilution, thatha i-0.3ml ye-dilution ngayinye. Xuba kahle no-0.2ml wesigaba se-logarithmic sensitive E. coli 226 bacteria suspension, bese wengeza i-LB half-body medium encibilikisiwe futhi yapholiswa yaba ku-45°C, xuba ngokushesha, bese uwuthela epuletini elimaphakathi eliqinile le-LB. Ngemva kokuqina, 37 Fukamela ku-℃ amahora angu-6 bese ubheka. Uma inani elikhulu lama-plaque livela epuletini elikhulisiwe, kufakazela ukuthi uhlobo oluhloliwe luyi-lysogen.
Ukuhlola ukuthi amagciwane ane-lysogenicity, landela lezi zinyathelo ezingenhla. Naka ubunyumba benqubo phakathi nokusebenza ukuze ugweme ukusebenza okungalungile okuthinta imiphumela yokuhlolwa.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.