English
Amaseli e-Sf9 angamaseli e-Spodoptera frugiperda, avame ukusetshenziselwa ukukhiqiza amaprotheni emithi ehlukahlukene namagciwane, noma ucwaningo oluyisisekelo. Lapho uhlakulela amaseli e-sf9, izinto ezisetshenziswa kakhulu ze-cell culture zithi ama-cell culture flasks.
Sf9 cells athathwe ku-Insect Pathology Laboratory yoMnyango Wezolimo wase-United States futhi athathwe kuseli IPLBSF-21 umugqa. Amaseli ajwayelekile anezinhlayiya futhi angakhuliswa ngokumiswa noma isiko lokunamathela. Leli ngqamuzana linezinga eliphezulu lokukhuluma (ikakhulukazi amaprotheni e-intracellular), linezinga lokukhula ngokushesha, ukugoqa amaprotheni okusebenzayo, i-glycosylation ifana namaseli ezilwane ezincelisayo, kanye ne-enzymatic deglycosylation elula (yokunqunywa kwesakhiwo samaprotheni) Ithandeka), isetshenziswa kabanzi enkulumweni. yama-V LPs.
Sf-9 amaseli ayimigqa yeseli enamathela kancane. Emkhakheni oqukethe u-10% we-serum yethole lengane, iningi lamaseli ngokuvamile likhula ngokunamathela, kanti ingxenye encane iyamiswa. Khula ku-27°C cell incubator ngaphandle kweCO2. Imvamisa ingaba yi-sub-flask ukuze idlule lapho ikhula ibe ukuminyana ngo-80-95%. Ukugaya i-trypsin akudingekile ngesikhathi sokuhamba. Ngokufaka amapayipi ngobumnene amaseli abambelelayo izikhathi ezi-5-10 nge-pipette engu-1 mL, amaseli angahlanjululwa futhi adlule ngemva kokungabambeleli. Ukucaciswa okuvamile kwama-flasks we-cell culture kuhlanganisa i-25cm2, 75cm2, 175cm2, 225cm2, njll. Ingaphezulu eliphathwa nge-TC lifanele isiko leseli elinamathelayo, futhi indawo ngaphandle kokwelashwa kwe-TC ifanele isiko leseli lokumiswa, elingahlangabezana nokukhula namasiko. izidingo zamaseli e-sf9. _
When performing cell passaging operations, it is recommended not to shake the cell culture flasks vigorously to avoid cell damage and affect the cell culture process.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.