English
Amaflask osiko lwamaseliabe nentamo eqondile, i-torticollis, i-engeli yentamo, unxantathu, unxande, njll. ngokwezimo ezihlukene. Imininingwane ihlanganisa 25ml, 75ml, 175ml, kanye 250ml. Osayizi abancane basetshenziselwa kakhulu ukudluliswa kwamaseli, ukulondolozwa kwamaseli, kanye nokuhlinzekwa kwamaseli okuhlolwa, njll. ukusetshenziswa.
Ngemva kokuba amaseli ekhule abe ungqimba olulodwa oluminyene kufulaski wosiko lweseli, empeleni egcwele. Ukuze unike amandla amaseli ukuthi aqhubeke nokukhula futhi andise inani lamaseli, kufanele abe ngaphansi kwe-subcultured (enziwe kabusha). I-Subculture nayo iyindlela yokulondoloza izinhlobo zamaseli. Ngesikhathi esifanayo, kuyinqubo edingekayo yokuhlolwa okuhlukahlukene kusetshenziswa amaseli akhulisiwe. Amaseli amisiwe angahlukaniswa ngokuqondile, kuyilapho amaseli anamathelayo adinga ukugaywa ngaphambi kokuba ahlukaniswe. Izinyathelo ezithile zimi kanje:
1. Lahla indlela yasekuqaleni yesiko kuflaski yeseli yesiko eligcwele amaseli.
2. Faka u-0.5-1ml 0.25% we-pancreatin solution, ukuze amangqamuzana angaphansi kwebhodlela acwiliswe esixazululo.
3. Faka isivalo senjoloba ebhodleleni bese usibeka ngaphansi kwesibonakhulu esihlanekezelwe ukuze ubuke amaseli. Ngokuhamba kwesikhathi, amangqamuzana anamathelayo okuqala kancane kancane aba yindilinga, futhi i-pancreatin yalahlwa lapho amangqamuzana engakantanti, kwase kwengezwa u-10ml we-culture medium ukuze kuqedwe ukugaya. Ukubuka ukugaya kungenziwa nangeso lenyama. Lapho phansi kwebhodlela kubonakala kumhlophe futhi kuvele amaphinifa amahle, ukugaya kungaqedwa. Ngokuvamile, isikhathi sokugayeka kokudla ezingeni lokushisa legumbi cishe imizuzu engu-1-3.
4. Sebenzisa i-pipette ukuze ushaye amangqamuzana anamathelayo abe yi-suspension, uwahlukanise abe amabhodlela amabili kuya kwamathathu, uxhume isisombululo se-culture ngesivalo senjoloba, bese usibeka ku-37 ° C ukuze uqhubeke nokuhlakulela. Qaphela ukukhula okubambelelayo ngosuku olulandelayo.
Okungenhla ukusetshenziswa kwe-amaflask e-cell culture ekudluliseni amaseli. Indlela yokulungiselela ikhambi lokugaya imi kanje: Kala i-trypsin engu-0.25g (umsebenzi 1:250), engeza u-100ml Ca2+, isixazululo se-Mg2+-free Hank's ukuze incibilike, hlunga Nzalisa ngokuhlunga, gcina ku-4°C, bese ubuyela ezingeni lokushisa elingu-37. °C ngaphambi kokusetshenziswa. I-EDTA ingaphinda yengezwe kwisisombululo se-pancreatin ukwenza ukugxila kokugcina kufinyelele ku-0.02%.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.