English
I-cell lysis iyindlela ebalulekile yokuhlola yokushintsha ukungena kweseli nomsebenzi. Kusho ukuthi ngemva kokuba ingqamuzana lingenwe igciwane, igciwane lisebenzisa izakhi ezisengqamuzaneni elibambayo ukuze lihlanganise inzalo yalo, bese inzalo yegciwane ibhubhisa ingqamuzana eliphethe futhi ikhishwe Ngaphandle kwamaseli. Ezivivinyweni ze-cell lysis, ama-cell culture flasks ziyinto esetshenziswayo evamile. Izinyathelo ze-cell lysis zimi kanje:
1. Thela i-culture medium, bese ubophela ibhodlela le-cellculture libheke phansi ephepheni elimuncayo ukuze iphepha elimuncayo limunce isiko (noma ubeke ibhodlela liqonde isikhashana ukuze uvumele insalela yesiko ukuthi igeleze iye phansi ebhodleleni. bese usebenzisa ipayipi ukulimunca ).
2. Engeza u-3ml ka-4℃ we-PBS epholile (0.01M pH7.2～7.3) ebhodleleni ngalinye lamaseli. Beka amaseli flat bese unyakazisa ngobumnene iminithi elingu-1 ukuze ugeze amaseli, bese ulahla isisombululo sokugeza. Phinda lokhu okungenhla kabili, ugeza amaseli kathathu ukuze ugeze uketshezi lwe-culture. Lahla i-PBS futhi ubeke flask yesiko eqhweni.
3. Engeza i-10ul PMSF (100mM) ku-1ml lysis buffer, qhaqhazela kahle futhi ubeke eqhweni. (I-PMSF kumele inyakaziswe kuze kungabikho amakristalu ngaphambi kokuxubana nesixazululo se-lysis.)
4. Engeza i-400ul ye-lysis buffer equkethe i-PMSF ku-flask ngayinye yamaseli, bese ulala eqhweni imizuzu engu-30. Ukuze umise ngokuphelele amaseli, iflaski kufanele inyakaziswe njalo.
5. Ngemuva kwe-lysis, sebenzisa i-scraper ehlanzekile ukuze usule amaseli ohlangothini olulodwa lweflask yesiko (isenzo kufanele sisheshe), bese usebenzisa isibhamu ukuhambisa udoti weseli bese u-lysate ku-1.5ml centrifuge tube. (Wonke umsebenzi kufanele wenziwe eqhweni ngangokunokwenzeka.)
6. I-Centrifuge ku-12000 rpm ku-4°C imizuzu emi-5. (Vula i-centrifuge kusengaphambili ukuze uphole kuqala)
7. Dlulisa i-centrifuged supernatant kushubhu le-centrifuge imizuzu engu-0.5 bese uyigcina ku- -20°C.
Amaflask empucuko yamaseli ayisetshenziselwa ukuhlolwa kwe-cell lysis kuphela, kodwa futhi namaseli asezingeni eliphakathi nesiko lezicubu elabhorethri, njengamaseli e-Vero. , amaseli we-HEK 293, amaseli e-CAR-T, amaseli e-CHO, amaseli ezinambuzane, njll.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.