Ukuhlakulela amaseli kuwumsebenzi onzima kakhulu. Ngokungeziwe ekudaleni indawo enhle yokukhula kwamaseli, kufanele futhi ixazulule ngokushesha izimo eziphuthumayo ezahlukahlukene phakathi nenqubo yesiko. Ukunqwabelana kweseli kokuthi ama-cell culture flasks kuyinto evame ukuhlangana nayo. Siyini isizathu salesi simo?
Isenzakalo sokunqwabelana kwamaseli kuflask ye-cell culture singahlaziywa ngokuvamile kusuka kulezi zici ezilandelayo:
1. Inqubo yokugaya ingase ibe nzima kakhulu, ipayipi liqine kakhulu, ukugaya ukudla isikhathi eside, ujusi wokugaya uqine kakhulu noma onobuthi, njll., kuzodala ukufa kwamangqamuzana. Ngemva kokufa kwengqamuzana, ikhipha i-DNA, ihlanganise amanye amangqamuzana, futhi yakhe iqoqo lamaseli anamathelayo.
2. Ijubane eleqile le-centrifugation yeseli noma isikhathi eside kakhulu kungabangela kalula ukunqwabelana kwamaseli.
3. Lapho ukugaya kungaphelele, ukuxhumana phakathi kwamaseli neseli akuhlukaniswa; lapho ukugaya sekuphelile, amangqamuzana ayindilinga avame ukunqwabelana futhi kuba nzima ukuhlukana futhi.
4. Amaseli asesimweni esibi nokuguga angase akhule abe yizigaxa (ezifana nesimo samaseli esingesihle esibangelwa ukubambezeleka ekushintsheni okuphakathi, okude kakhulu ukuthi kungadlula, ukungcola, njll.).
5. Akukho ukushaywa kwamapayipi ngokulinganayo lapho udlula, futhi maningi ama-cell clumps, azoba yimfucuza lapho ekhuliswa.
6. Ukujova okungalingani noma amaseli angagayeki.
7. Isiko lokungadlidlizi noma amagciwane amaningi (amaseli) azokwakha ama-clumps.
Uma amaseli kokuthi amaflaksi amasiko eseli ebonakala ehlangene, ungakwazi ukuhlaziya izici ezingenhla ukuze uthole isizathu, futhi wenze izinqubo eziqinile ngaphambi kokuhlinzwa ukuze uzigweme. lesi senzo.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.