English
Hayotiy fanlar sohasining chuqur rivojlanishi bilan hujayra madaniyati texnologiyasi biofarmatsevtika, sanoat vaktsina ishlab chiqarish va monoklonal antikorlar kabi ko'plab sohalarda keng qo'llaniladi. Hujayra o'sishining turli usullariga ko'ra, u ikki toifaga bo'linadi: yopishgan hujayralar va suspenziya hujayralari. Shunday qilib, hujayra fabrikalari yordamida qaysi hujayralarni etishtirish mumkin?
Hujayra zavodi hujayra madaniyati sarf materiallarining ko'p qatlamli tuzilishidir. Umumiy spetsifikatsiyalar 1 qatlam, 2 qatlam, 5 qatlam, 10 qatlam, 40 qatlam va boshqalarni o'z ichiga oladi. U asosan yopishgan hujayralar madaniyati uchun ishlatiladi. Yopishqoq hujayralar o'sishi biriktirilishi mumkin bo'lgan tayanch yuzasiga ega bo'lishi kerak va hujayralar o'zlari tomonidan ajratilgan yoki madaniy muhitda ta'minlangan biriktiruvchi omillarga tayangan holda bu sirtda o'sishi va ko'payishi mumkin. Hujayralar biriktirilgandan so'ng, ular odatda bir necha kun ichida madaniyat yuzasini qoplaydi va Vero hujayralari, HEK 293 hujayralari, CAR-T hujayralari, MRC5, CEF hujayralari, cho'chqa alveolyar makrofaglari, miyelom hujayralari, DF kabi zich hujayra monoqatlamini hosil qiladi. 1 hujayra, ST xujayralari, PK15 xujayralari, Marc145 xujayralari va boshqalar madaniyat uchun hujayra zavodiga yopishtirilgan.
Bundan tashqari, bu sarf materialdan suspenziya hujayralarining statsionar madaniyati uchun ham foydalanish mumkin. Suspenziya xujayralari tayanch yuzasidan mustaqil ravishda o'sadigan va limfotsitlar kabi madaniy muhitda suspenziyada o'sadigan hujayralarga tegishlidir.
Hujayra fabrikalari odatda polistirol xom ashyosidan ishlab chiqariladi va sirt hidrofobikdir. Agar u yopishgan hujayra madaniyati uchun ishlatilsa, uning gidrofilligini oshirish uchun sirtni TC bilan davolash kerak, bu hujayra yopishgan o'sishi uchun mos keladi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.