Hujayra madaniyati plastinkasi kichik o'lchamdagi hujayra madaniyati uchun sarflanadigan asosiy madaniyat hisoblanadi. Foydalanish jarayonida biz ko'pincha hujayralar to'planishi va notekis taqsimlanishi fenomeniga duch kelamiz. Bu holatni qanday hal qilish mumkin?

Hujayra kulturasi plastinkasida hujayralarning notekis taqsimlanishiga duch kelganda, avvalo hujayralar qanday aralashganiga e'tibor bering, u pipetlash yoki kultura plastinkasini silkitish. Agar u ikkinchisi bo'lsa va u aylana bo'ylab silkitsa, bu markazdan qochma kuchga bog'liq bo'lishi mumkin. Hujayralar periferik qismga tashlanadi, natijada hujayralar o'rtasida to'rt haftadan kamroq vaqt qoladi. Urug'lik plastinkasini etishtirishdan oldin, kultura plitasini bir necha soat to'yinganligi uchun inkubatorga qo'ying va keyin uni chiqarib oling. Hujayralarni emlashda kuch engil bo'lishi kerak. Plitalar quduqlariga hujayra suspenziyasini oqishini ta'minlash uchun plastinka qudug'ining yon tomoniga asta-sekin qo'shish uchun tomchilatib qo'yishingiz mumkin, shunda kulturali hujayralar asosan bir tekisda o'sadi. Hech qachon tebranish uchun osilatordan foydalanmang, aks holda hujayralar to'planib qoladi.

Madaniyat plastinkasi teshigining diametri qanchalik kichik bo'lsa, hujayralarning notekisligi shunchalik aniq bo'ladi. Bu hodisa 24 quduqli va 96 quduqli plitalarda ko'proq uchraydi, chunki suyuqlik devori quduqdagi madaniy muhitni suyuqlik tekisligi emas, balki bu ikki quduq plitasidan foydalanish ehtiyojdan kelib chiqadi. aralashuv va boshqa sabablarga ko'ra, madaniyat muhitini etarli miqdorda qo'shish mumkin emas, shuning uchun hujayralar madaniyat muhiti bilan "chekka yig'ish" paydo bo'ladi. Quduq markazida bir xil hujayralar bo'lishi uchun emlangan hujayralar soni ko'paytirilsa, qanday ko'rsatkichlarga rioya qilish kerak, MTT bo'lsa, Immunogistokimyo hujayralarning qatlamlanishi tufayli natijalarga ta'sir qiladi.

Hazm qilganda. hujayralar, hujayra to'planishiga yo'l qo'ymaslik uchun teng ravishda pipetirovka qilishga e'tibor bering va hujayra madaniyati plastinkasi quduqlarida madaniyat muhiti miqdori etarli bo'lishi kerak. Umuman olganda, emlash paytida etarli madaniyat muhitini qo'shing va aralashuvni qo'shganda muhitni bir marta o'zgartiring. Interventsiya muhiti va madaniy muhit miqdori emlash bilan teng Bu holda “edge collection” fenomeni yaxshilanadi.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.