English
Keng miqyosli hujayra madaniyatida hujayra fabrikalari keng tarqalgan ishlatiladigan sarf materiallari bo'lib, ular asosan yopishgan hujayralar madaniyati uchun ishlatiladi. Hujayra o'sishi turli xil oziq moddalarni talab qiladi, shuning uchun ular nima?
1. Madaniyat muhiti
Hujayra ishlab chiqarish muhiti hujayra zavodidagi hujayralarni o'sishi uchun zarur bo'lgan ozuqa moddalari, jumladan uglevodlar, aminokislotalar, noorganik tuzlar, vitaminlar va boshqalar bilan ta'minlaydi. Turli hujayralarning ozuqaviy ehtiyojlariga ko'ra, turli xil sintetik moddalar mavjud. EBSS, Eagle, MEM, RPMll640, DMEM va boshqalar kabilardan tanlash uchun media.
2. Boshqa qo'shimchalar
Turli xil sintetik vositalar tomonidan ta'minlangan asosiy oziq moddalarga qo'shimcha ravishda, sarum va omillar kabi boshqa komponentlar turli hujayralar va turli madaniyat maqsadlariga ko'ra qo'shilishi kerak.
Sarum hujayradan tashqari matritsa kabi muhim moddalarni beradi. , o'sish omillari va transferrin va homila sigir zardobi odatda qo'llaniladi. Qo'shilgan sarumning nisbati turli hujayralar va turli tadqiqot maqsadlariga ko'ra aniqlanishi kerak. 10% ~ 20% sarum hujayralarning tez o'sishi va ko'payish tezligini saqlab turishi mumkin, bu o'sish muhiti deb ataladi; hujayralarning sekin o'sishi yoki o'lmasligini ta'minlash uchun 2% ~ 5% sarum qo'shilishi mumkin, bu parvarishlash muhiti deb ataladi.
Glutamin hujayra o'sishi uchun muhim azot manbai bo'lib, hujayra o'sishi jarayonida muhim rol o'ynaydi. va metabolizm. Biroq, glutamin juda beqaror va eritmada oson parchalanishi sababli, glutaminning taxminan 50% 4 ° C da 7 kundan keyin parchalanishi mumkin. Ishlatishdan oldin aminoamid qo'shilishi kerak.
Umuman olganda, hujayra madaniyati uchun turli xil muhitlar va sarum ishlatiladi, ammo kultura paytida hujayralar ifloslanishini oldini olish uchun penitsillin, streptomitsin, gentamitsin kabi ma'lum miqdorda antibiotiklar, va boshqalar, shuningdek, madaniy muhitga qo'shiladi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.