English
Hujayra kulturalariasosan kichik va oʻrta miqyosli hujayra kulturalarida qoʻllaniladi, shuning uchun hujayra madaniyati kolbalarida qanday hujayralarni etishtirish mumkin?
Hujayralarning koʻp turlari mavjud boʻlib, ular yopishgan hujayralar va suspenziya hujayralariga boʻlinadi. ularning o'sish shakllariga ko'ra. Yopishqoq hujayralarning o'sishi biriktirilishi mumkin bo'lgan tayanch yuzasiga ega bo'lishi kerak va hujayralar o'zlari tomonidan chiqariladigan yoki madaniy muhitda ta'minlangan biriktiruvchi omillarga tayanadi. Bu sirtda o'sishi va ko'payishi uchun. Suspenziya hujayralarining o'sishi tayanch yuzasiga bog'liq emas va madaniy muhitda suspenziyada o'sadi. Hujayra kulturalari odatda shaffof polistirol xom ashyosidan tayyorlanadi. Umumiy xususiyatlarga 25cm2, 75cm2, 175cm2 va 225cm2 kiradi. Hujayra kolbasining pastki yuzasi hujayralarni pipetlashda aloqa o'lik burchaklarini kamaytirish uchun yoy shaklida yaratilgan. U yopishgan hujayralar madaniyati uchun ham, suspenziya hujayralarining statik madaniyati uchun ham qo'llanilishi mumkin. Qo'llaniladigan hujayra turlari quyidagilardir:
Birikuvchi hujayralar: Vero hujayralari, HEK 293 hujayralari, CAR-T hujayralari, MRC5, CEF hujayralari, cho'chqa alveolyar makrofaglari, miyelom hujayralari, DF-1 hujayralari, ST hujayralari, PK15 hujayralari, Marc145 hujayralar va boshqalar.
Suspension hujayralar: CHO hujayralari, hasharotlar hujayralari, BHK21 hujayralari va MDCK hujayralari va boshqalar.
Shuni ta'kidlash kerakki, agar hujayra kulturalari yopishgan hujayralarni etishtirish uchun ishlatilsa, uning yuzasi yopishgan hujayralarning o'sish xususiyatlarini qondirish uchun uning gidrofilligini oshirish uchun kolbani maxsus o'zgartirish kerak. Suspension hujayra kulturasida foydalanilganda maxsus ishlov berish talab etilmaydi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.