English
Hozirgi vaqtda yopishgan hujayralar hujayra madaniyatining asosiy turi hisoblanadi. Ular tayanch yuzasida adsorbsiyalanishi va sirtda o'sishi va ko'payishi uchun o'zlari tomonidan ajratilgan yoki madaniy muhitda ta'minlangan biriktiruvchi omillarga tayanishi kerak. Ba'zan biz hujayra madaniyati flasks dagi hujayralar devorga yopishib ololmasligini aniqlaymiz. Bunga nima sabab bo'lmoqda?
Hujayra yopishishi atrof-muhitga moslashish reaktsiyasidir. Madaniy muhitdan kattaroq zichlikka ega bo'lgan hujayralar pastki yuzaga joylashadi. Hujayralar omon qolishi uchun ular bu muhitni yaxshilashlari, hujayradan tashqari matritsani ajratishlari, pastki sirt tuzilishini yaxshilashlari va keyin tabiiy ravishda pastki yuzaga yopishishi kerak. Tepasida. Zichligi suvdan kam bo'lgan hujayralar suyuqlik yuzasida suzib yuradi, shuning uchun uning pastki yuzasiga yopishib olish mumkin emas, lekin agar kultura eritmasi hujayralar hujayra kulturalarining yuqori devoriga tegishi uchun to'ldirilgan bo'lsa, ular devorga ham yopishib oling. Agar hujayralar devorga yopishmasa, uni quyidagi jihatlardan tahlil qilish mumkin:
1. Hujayralarning o'tishi uchun eng muhim narsa tripsin bilan davolash vaqtidir: hujayralar o'zlari etarli darajada hazm bo'lmasa, aglomeratsiya qilinadi; tripsin bilan davolash juda uzoq bo'lsa, hujayra membranasi oqsilining shikastlanishiga olib kelishi oson, hujayralar devorga mahkam yopishmasligi va mikroskop ostida kuzatuvning uch o'lchovli ta'siri kuchli emas. , Va hatto og'ir holatlarda hujayra o'limiga olib keladi.
2. Adezyon omillarining etishmasligi: sarumda adezyonni kuchaytiruvchi omillar mavjud va zardobsiz muhit jarayonida bunday yopishqoqlikni kuchaytiruvchi omillarning etishmasligi hujayralarning yopishish ta'sirini sezilarli darajada kamaytiradi.
3. Mikoplazma yoki bakteriyalarning ifloslanishi.
4. Haddan tashqari gidroksidi pH va juda kam Gln.
5 kabi kultura eritmasining noto'g'ri konfiguratsiyasi va saqlanishi. Qayta tiklangan hujayralar yaxshi holatda emas, ular qariydi va yopishqoqligini yo'qotadi.
6. Emlangan hujayralar soni hujayradan tashqari matritsani ajratish uchun juda kichik.
7. Reanimatsiya paytida ishlov berish tezligi juda sekin va reanimatsiya jarayoni noto'g'ri.
Birgalikda olib qaraganda, yopishgan hujayralarning yopishmasligi asosan yuqoridagi sabablarga bog'liq. Bundan tashqari, sirtda TC bilan ishlov berishdan o'tmagan plastmassa hujayra madaniyati kolbalari hujayralarning yopishish ko'rsatkichlariga ham ta'sir qiladi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.