English
- bu shisha turidagi hujayra madaniyatining bir turi. Hujayralarning yopishqoqligini oshirish uchun, birlamchi hujayralarni o'stirishda sarflanadigan materiallar qoplanadi. Odatda ishlatiladigan qoplama buferi quyidagilarni o'z ichiga oladi:Polilizin: Bu sintetik birikma, musbat zaryadlangan aminokislota bo'lib, kultura substrati yuzasida elektrodni o'zgartirib, hujayra yopishishini ta'minlaydi. Hujayra madaniyatida hujayra yopishishini rag'batlantirish uchun qoplamalar uchun keng qo'llaniladi. Hujayra yopishishi va o'sishini rag'batlantirishdan tashqari, qoplama eritmasi bilan polilizin qoplamasi birlamchi hujayralarning omon qolish darajasini oshirishi va neyritning o'sishini rag'batlantirishi mumkin.hujayra kulturasi
Fibronektin: Bu yuqori molekulyar glikoprotein bo'lib, 1974 yilda chet elda tadqiq qilinib, kashf etila boshlandi. , va u turli xil biologik funktsiyalarga ega. Fibronektin molekulyar og'irligi 440-500 kDa bo'lgan glikoproteindir. U hujayra yuzasi oqsillari va plazmasida mavjud. U hujayra membranasining sirt retseptorlari va hujayradan tashqari matritsa komponentlari bilan birlashib, hujayra yopishishi va kengayishi o'sishini rag'batlantiradi.
Jelatin: kollagendan olingan yuqori molekulyar og'irlikdagi suvda eriydigan oqsillar aralashmasi, 0,2% qoplama suyuq jelatin eritmasi madaniy tomirlarni qoplash uchun ishlatiladi. hujayra yopishishini rag'batlantirish. Differensiatsiyalash uchun inson embrion ildiz hujayralarini, ma'lum birlamchi hujayralarni va o'lmas hujayralarni kiriting.
Yuqoridagilar hujayra madaniyati kolbalarida keng qo'llaniladigan bir nechta qoplama buferidir. Qoplama buferining o'ziga xos tanlovi hujayralarning turiga va xususiyatlariga bog'liq. Ammo shuni ta'kidlash kerakki, shishani qoplash va ishlatish oralig'i bir kundan oshmasligi kerak. Qoplangan idish qancha uzoq saqlansa, uning yopishish ko'rsatkichi ta'sir qiladi.
The above are several coating buffer commonly used in cell culture flasks. The specific choice of coating buffer depends on the type and characteristics of the cells. However, it should be noted that the interval between coating and use of the bottle should not exceed one day. The longer the coated container is stored, its adhesion performance will be affected.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.