English
Biofarmatsevtika, vaktsinalar va monoklonal antikorlar kabi sohalarning jadal rivojlanishi bilan keng miqyosli hujayra madaniyatida hujayra fabrikalari ning ahamiyati tobora ortib bormoqda. Hujayra madaniyati qattiq ish bo'lib, hujayra o'sish muhiti, madaniyat muhiti qo'shilishi va hujayralar hosilini qattiq nazorat qiladi.
Hujayra zavodidan hujayralarni yig'ish uchun foydalanilganda quyidagi tartib-qoidalar bajarilishi kerak. qat'iy rioya qilinadi:
1. Inkubatsiyadan keyin kultura eritmasini to'kib tashlang va uni kaltsiysiz va magniysiz fosfat buferi (CMF-PBS) (40-50 ml / qatlam) bilan yuving va agar kerak bo'lsa, yuvishni takrorlang.
2. Ovqat hazm qilish: hazm qilish eritmasi (10-40 ml / qatlam) oldindan qizdiriladi.
3. Yig'ish: hazm qilish eritmasini olib tashlash va hujayralarni yig'ish uchun 5 daqiqa davomida 1000 aylanish tezligida santrifüj qiling.
4. Yuvish: hazm qilingan inkubatorni CMF-PBS yoki madaniy muhit bilan yuving.
Hujayralarni yig'ishda quyidagi nuqtalarga e'tibor bering:
1. CMF-PBS har bir kultura yuzasiga toʻliq botirilganligiga ishonch hosil qiling va qolgan kultura suyuqligini tozalash uchun hujayra zavodini oldinga va orqaga muloyimlik bilan silkiting.
2. Ovqat hazm qilish eritmasi har bir qatlamga teng ravishda taqsimlanadi. Ovqat hazm qilish eritmasi kultura yuzasiga to'liq botirilishini ta'minlash uchun inkubatorni sekin oldinga va orqaga burang. Hujayralar sirtdan ajralib chiqishiga yordam berish uchun inkubatorga sekin teging.
3. Hujayra fabrikasi uchun o'rta qatlamdagi hujayralarning hazm qilish holatini aniq kuzatish mumkin emasligi sababli, bir xil madaniyat sharoitida kultura kolbasi yoki bir qatlamli hujayra zavodining hazm qilish holatiga murojaat qilish tavsiya etiladi. Har bir hujayra qatlamining o'sish holatini kuzatish uchun maxsus ko'p qatlamli hujayra madaniyatini kuzatish platformasidan ham foydalanishingiz mumkin.
3. Agar tozalash eritmasida yoki hujayra zavodining kultura qatlamida hujayralar ko'p bo'lsa, uni bir necha marta tozalash yoki hujayra hazm qilish ishini sozlash kerak.
4. Madaniyat haroratining ozgina og'ishi hujayralarni yig'ish tezligiga ta'sir qilishi mumkin, shuning uchun madaniyat haroratiga diqqat bilan e'tibor bering belgilangan harorat.
Yuqorida cell zavodi dagi hujayralarni yig'ish jarayoni va ehtiyot choralari ko'rsatilgan. Hujayralar atrof-muhitga juda sezgir. Hujayralarni yig'ishda yaxshi ish qilish hujayra madaniyati muvaffaqiyatining kalitidir va barcha aloqalarni qattiq nazorat qilish kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.